云药七龙天对缺氧大鼠动脉血气及肺组织中血管内皮生长因子蛋白表达的影响

Effects of Yunnan Ethnic Medicine Qilongtian Compound on Arterial Blood Gas and Protein Expressions of VEGF in Lung Tissues of Aanoxic Rats

目的观察七龙天对缺氧大鼠动脉血气及肺组织中血管内皮生长因子(VEGF)蛋白表达的影响,为探寻云南民族医药防治慢性缺氧性肺病的有效手段提供实验依据。方法将SD大鼠40只随机分为5组,即正常对照组、模型组及七龙天低、中、高剂量组,每组8只,除正常对照组以外均以常压低氧法制备大鼠缺氧模型。给药组分别于造模第1日始灌胃相应剂量的七龙天,连续用药3周。腹主动脉采血行血气分析,采用蛋白免疫印迹法检测肺组织中VEGF的蛋白表达。结果与正常对照组比较,模型对照组大鼠血气分析比较差异无统计学意义(P>0.05),VEGF蛋白表达水平明显升高(P<0.05);与模型对照组比较,七龙天中、高剂量组大鼠VEGF的蛋白表达水平明显降低,以高剂量组最为显著(P<0.05)。结论七龙天可显著抑制缺氧诱导血管新生形成。

Objective To observe the effects of Qilongtian compound on arterial blood gas and protein expressions of VEGF in the lung tissue of anoxic rats,and provide experimental evidence of searching for effective method from Yunnan ethnic medicine to prevent and cure chronic anoxic lung disease.Methods Forty SPF grade SD rats were randomly divided into 5 groups,with 8 in each,namely,normal group,model group,low dose of Qilongtian group,medium dose of Qilongtian group,high dose of Qilongtian group.Anoxic model was established by normbaric hypoxia except for normal control group.Corresponding medicines were administered by gavage for 3 weeks since the first day of modeling.Arterial blood gas analysis was conducted after drawing blood from the abdominal aorta.The protein expression of VEGF was detected by protein blotting and immunoassay.Results Compared with normal group,the arterial blood gas of model group had no significant change（P0.05） and the level of protein expression of VEGF in the lung tissue increased significantly（P0.05）.Compared with model group,the level of protein expressions of VEGF were reduced in medium dose and high dose of Qilongtian group,and that of high dose group was significant（P0.05）.Conclusion Qilongtian compound can inhibit angiogenesis induced by anoxia.