摘要
目的观察抵挡汤早期干预对2型糖尿病大鼠血管病变中大血管的保护作用,探讨其机制。方法采用高脂饲料喂养及链脲佐菌素诱导方法制成大鼠糖尿病模型,抵挡汤早期干预组、抵挡汤中期干预组和抵挡汤晚期干预组分别于实验成模前4周、成模和成模后4周给予抵挡汤灌胃,辛伐他汀组和罗格列酮组大鼠于成模时分别给予辛伐他汀和罗格列酮灌胃,至实验24周时结束。酶联免疫吸附法检测大鼠血清血管细胞黏附分子-1(VCAM-1)水平,实时荧光定量PCR法检测主动脉肿瘤坏死因子-α(TNF-α)mRNA表达。结果与正常对照组比较,模型对照组大鼠血清VCAM-1水平明显升高(P<0.05),主动脉TNF-αmRNA表达明显升高(P<0.05);与模型对照组比较,抵挡汤早期干预组和辛伐他汀组大鼠血清VCAM-1水平明显降低(P<0.05),并且抵挡汤早期干预组、抵挡汤中期干预组和罗格列酮组大鼠主动脉TNF-αmRNA的表达明显降低(P<0.05)。结论抵挡汤早期干预能抑制大鼠主动脉TNF-αmRNA表达升高,抑制大血管病变的炎性损伤,延缓糖尿病大血管病变的发展,发挥其保护大血管的作用。
Objective To observe the protective effects of the early intervention with Didang decoction on the experimental type 2 diabetic rats with macrovascular lesions.Methods Experimental diabetic rats were induced by high fat diet and streptozotocin.The rats in Didang decoction late group,Didang decoction middle group and Didang decoction early group were given Didang decoction from 4 weeks before modeling,modeling completed and 4 weeks after modeling respectively to 24th week.The rats in simvastatin group and rosiglitazone group were given simvastatin and rosiglitazone respectively from modeling completed to 24th week.The level of VCAM-1 in serum was detected by ELASA and the mRNA expression of TNF-α in aorta using real-time PCR.Results Compared with normal control group,the level of VCAM-1 in serum and the mRNA expression of TNF-α in aorta were higher in model control group（P0.05）.Compared with model control group,the level of VCAM-1 in serum was lower in Didang decoction early group and simvastatin group（P0.05）,the mRNA expression of TNF-α in aorta was lower in Didang decoction early group,Didang decoction middle group and rosiglitazone group（P0.05）.Conclusion The level of VCAM-1 in serum was reduced and the mRNA expression of TNF-α in aorta of experimental type 2 diabetic rats was inhibited after early intervened with Didang decoction.Thus the inflammatory injury of macrovascular lesions was inhibited,the development of diabetic vascular disease was delayed,and the great vessels was protected.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第10期38-40,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(30973753)
关键词
抵挡汤
糖尿病
大血管病变
肿瘤坏死因子-Α
血管细胞黏附分子-1
大鼠
Didang decoction
diabetes mellitus
macrovascular disease
TNF-α
VCAM-1
rats HPLC WANG Xiao-lin1
2
ZHANG Ping3
GUO Min2
LI Xiao-dong2
JIANG Hua2（1.Lanzhou University of Technology
Lanzhou 730050
China
2.Gansu Provincial Hospital of TCM
Lanzhou 730050
China
3.Gansu Institute for Food and Drug Control
Lanzhou 730050
China） Abstract：Objective To establish the method for simultaneous determination of panax notoginseng saponins R1
ginseng saponins Rg1
ginseng saponins Rb1 in Longzhong Sunshang Capsule by HPLC with gradient elution.Methods C18 column（250 nm×4.6 nm
5 μm） was used with mobile phase of acetonitrile（A）-0.05% phosphoric acid solution（B）
gradient elution 0-32 min（A 23%-23%）
32-42 min（A 23%-30%）
42-67 min（A 30%-46%）.The detection wavelength was 203 nm
and the flow rate was 1.0 mL/min.Results The linear relationship of panax notoginseng saponins R1
ginseng saponins Rg1
ginseng saponins Rb1 was in the range of 1.02-8.50 μg（r=0.999 5）
0.90-7.50 μg（r=0.999 0） and 1.17-9.75 μg（r=0.999 6） respectively.The average recovery was 100.98%（RSD=2.71%）
97.73%（RSD=1.98%） and 99.57%（RSD=1.62%）.Conclusion The method is simple
accurate
reliable
and can be used for quality control of Longzhong Sunshang Capsule. Key words：Longzhong Sunshang Capsule
panax notoginseng saponins R1
Ginseng saponins Rg1
Ginseng saponins Rb1
content determination
