高效液相色谱法测定马兰中大黄酸、大黄素及大黄酚的含量

Determination of Rhein,Emodin,and Chrysophanol in Kalimeris Indica by High Performance Liquid Chromatography

目的建立马兰药材中大黄酸、大黄素及大黄酚含量的测定方法。方法采用高效液相色谱法(highperformance liquid chromatography,HPLC)。色谱柱:Kromasil-C18柱(250mm×4.6mm,5μm);流动相:甲醇-0.1%磷酸水溶液;梯度洗脱;检测波长:254nm;流速:1ml/min;柱温:30℃。结果大黄酸、大黄素、大黄酚浓度分别在0.253～2.530μg/ml(r=0.999 6)、0.249～2.490μg/ml(r=0.999 3)、0.257～2.570μg/ml(r=0.999 7)范围内与峰面积呈良好的线性关系;平均加样回收率(n=6)分别为99.27%(RSD=1.28%)、99.78%(RSD=1.05%)、99.92%(RSD=0.83%)。结论所建立的方法准确、可靠、简便,适合马兰药材中大黄酸、大黄素及大黄酚含量的测定。

Objective To establish a method for determining the contents of rhein, emodin, and chry- sophanol in Kalirneris indica by high performance liquid chromatography （HPLC）. Methods HPLC was performed on Kromasil C18 column （250mm×4.6mm,5μm） with a mobile phase of methanol-0.1G phos-phoric acid for gradient elution at a detection wavelength of 254 nm,a flow rate of 1 ml/min,and a column temperature of 30℃. Results The concentrations of rhein,emodin,and chrysophanol showed a good linear relationship with peak areas in 0. 253-2. 530 μg/ml （r=0. 9996）, 0. 249-2. 490 μg/ml （r=0. 9993）, and 0. 257 2. 570μg/ml （r=0. 9997）, and the average recovery rates （n=6） were 99.27% （RSD=1. 28%） 99. 78%o （RSD= 1.05 %）, and 99.92%（RSD= 0.83%）, respectively. Conclusion HPLC determination method can be used for measuring the contents of rhein, emodin, and chrysophanol in Kalimeri,s indica for its accuracy, reliability, and simplicity.