地塞米松磷酸钠诱导后雌性大鼠血浆中氨苯砜含量的测定

Determination of Dapsone in the plasma of female rats induced by Dexamethasone sodium phosphate

目的建立测定经地塞米松磷酸钠诱导后雌性大鼠血浆中细胞色素P450 3A4(CYP3A4)探针药物氨苯砜浓度的方法。方法采用高效液相色谱法,色谱柱为Ultimate-XB C18(250 mm×4.6mm i.d.,4μm),流动相为乙腈—水(25:75),流速为1.0ml/min,紫外检测波长为258nm,柱温为30℃,进样量为30μl。结果氨苯砜血药浓度在1.024～10.24mg/L范围内线性关系良好(r=0.9935),方法回收率为97.53%～99.52%,日内、日间相对标准偏差(RSD)分别为1.2%～2.3%、2.8%～4.9%。结论本方法快速、准确、灵敏度高、专属性强,适用于大鼠血浆中氨苯砜浓度的测定。

Objective To establish the method for determination of dapsone concentration which is the probe drug of cytochrome P450 3A4 （CYP3A4） in the serum. Methods Using high performance liquid chromatography（ HPLC）, the chromatographic column was the Ultimate-XB C18 （250mm × 4.6mm id,4μm）, the mobile phase was acetonitrile-water （25：75）, the flow rate was 1.0ml/min, the UV detection wavelength was 258 nm, the column temperature was 30℃, the injection volume was 30μl. Results The plasma concentration of dapsone in the range of 1. 024～10.24mg/L showed a good linear relationship （r = 0. 9935）. Method recoveries were 97.53% ～ 99.52%, intro-day and inter-days RSD were 1. 2 ～ 2.3%, 2. 8 ～ 4.9%. Conclusion This method was rapid, accurate, high sensitivity, strong specificity, and suitable for determination of dapsone concentrations in serum.