Spectrophotometric Error Due to Uncertainty in Reading the Signal

Based upon the standard deviations for the intercept and the slope of a linear regression line, as well as by differentiating both Beer＇s law and a linear relationship between transmittance and concentration at high transmission, it is proved that the relative spectrophotometric error of a measurement becomes greater as the sample concentration only decreases. Further, it is demonstrated that the present knowledge with regard to the error in absorption spectrophotometry is necessary to be reexamined. The total scale of transmittance can literally be used for measurements, unfolding workable dynamic ranges about two orders of magnitude lower than usually and thus absorption spectrophotometry can efficiently compete with other methods of analysis with respect to detection limits.