犬瘟热病毒N蛋白抗原表位基因的合成和杆状病毒表达载体的构建

Synthesis the Gene Including the N Protein Antigen Epitopes of CDV and Construction the Baculovirus Expressive Vector

目的串联合成犬瘟热病毒N蛋白抗原表位基因,并构建其杆状病毒表达载体。方法利用生物信息学软件,预测N蛋白的抗原表位,串联合成这些抗原表位。利用定向克隆的方法,将该基因片段连接到杆状病毒表达载体pFastBac-Hta。结果获得了包含犬瘟热病毒N蛋白抗原表位的基因,并成功构建pFastBac-N合成表达载体。结论该实验为犬瘟热病毒N蛋白抗原表位基因在杆状病毒内的表达奠定了基础。

Objective To synthesize the gene including the CDV-N protein antigen epitopes,and construct the baculovirus expression vector.Methods The N protein antigen epitopes was predicted by bioinformatics software and synthesized tandem.The fragment was cloned into the baculovirus expression vector pFastBac-Hta.Results The gene including the CDV-N protein antigen epitopes was obtained and successfully constructed the pFastBac-Nsynthesis expression vector.Conclusion The experiment laid the foundation for the CDV-N protein antigen epitopes expression in baculovirus.