大鼠经皮给药血浆中多奈哌齐浓度的UPLC-MS/MS法测定

Determination of Donepezil in Rat Plasma after Transdermal Administration by UPLC-MS/MS

建立了超高效液相色谱-串联质谱法测定大鼠血浆中的多奈哌齐。以氯雷他定为内标,液-液萃取处理血浆样品,采用ESI源正离子模式、多反应监测进行定量分析。监测离子对为m/z 380.3→m/z 243.2(多奈哌齐)和m/z 383.1→m/z337.1(氯雷他定)。多奈哌齐在0.5～200 ng/ml范围内线性关系良好,方法回收率为94.1%～106.8%,日内、日间RSD均小于10%。

An UPLC-MS/MS method was established for the determination of donepezil in rat plasma with loratadine as the internal standard. The plasma samples were pretreated by liquid-liquid extraction. The mass spectrometer was used in the multiple reaction monitoring （MRM） with the ESI-positive mode. The MRM was performed at m/z 380.3→m/z 243,2 for donepezil and m/z 383.1→m/z 337.1 for loratadine. The calibration curve of donepezil was linear in the concentration range of 0.5 - 200 ng/ml. The method recoveries were between 94.1% - 106.8 %. The intra- and interday RSDs were less than 10 %.