星点设计-效应面法优化碎米糖化液的脱色工艺研究

Optimization of Decolorization Technology of Saccharification Liquid from Broken Rice by Central Composite Design-response Surface Methodology

目的:采用星点设计-效应面法优化碎米糖化液脱色的工艺条件。方法:以脱色剂活性炭用量、pH值和加热温度为考察因素,以葡萄糖保留率和脱色率为效应值,分别采用多元线性回归和二项式方程拟合,用效应面法优化工艺,制备3批样品并进行验证试验。结果:碎米糖化液活性炭脱色的优化工艺为糖化液加入4%的活性炭后,在pH值3.5～4.5、60℃下脱色,3批样品保留率和脱色率预测值与实测值的平均偏差分别为(5.16±0.12)%、(7.13±0.21)%。结论:本文所建立的脱色工艺简便可行,可为进一步制备注射用葡萄糖的脱色工艺研究提供试验依据。

OBJECTIVE： To optimize the decolorization technology of saccharification liquid from broken rice by central com- posite design and response surface methodology. METHODS： The decolorization technology was optimized by response surface methodology （RSM） with dosage of decoloring agents, pH value and heating temperature as factors using the retain rate and the de- coloring rate of glucose as index. The multiple linear regression and binomial regress equation were fitted respectively, and valida- tion test of 3 batches of samples was carried out. RESULTS ： The optimum decolorization conditions of saccharification liquid from broken rice were 4% active carbon, pH value of 3.5-4.5, at 60 ℃. Average deviation of predicted value and measured value of re- tention rate and decoloring rate were （5.16 ±0.12）% and （7.13±0.21）% in 3 bathes of samples. CONCLUSION： The decoloriza- tion technology is simple and practicable. It can provide experimental basis for further study on decoloring technology of Glucose for injection.