乌参醒脑方对大鼠脑缺血致血管性痴呆的神经保护作用

Neuroprotective effect of Wushen-Xingnao Fang against neuronal injury and vascular dementia induced by transient forebrain ischemia in rats

目的:考察乌参醒脑方(WSXN)对大鼠脑缺血致血管性痴呆的神经保护作用。方法:大鼠随机分为假手术组、模型组、WSXN低、中、高剂量组(28,56,112 mg·kg-1)和EGB761阳性对照组(80 mg.kg-1),每组10只;采用改良的Pulsinelli四血管阻断法制备全脑缺血-再灌注损伤学习记忆的大鼠血管性痴呆模型。灌胃给药20 d(预防6 d+治疗14 d),对照组给予等容量溶媒。采用Morris水迷宫定向航行试验及空间探索试验检测各组大鼠的学习记忆能力,光镜观察脑海马神经组织及细胞形态学变化。结果:大鼠全脑缺血再灌注引起脑海马神经细胞损伤和学习忆力能力损害;中、高剂量WSXN和EGB761能剂量依赖性地显著增加脑缺血后大鼠海马幸存的活锥体神经元密度和尼氏体数目,还能改善血管性痴呆大鼠的空间学习记忆和参照记忆能力。结论:乌参醒脑方对全脑缺血再灌注神经损伤和血管性痴呆有显著的治疗作用。

Objective： To investigate the neuroprotective effect of Wushen-Xingnao Fang （WSXN） against neuronal injury and vascular dementia （VD） in forebrain ischemic rats. Methods： After transient forebrain ischemia/reperfusion was induced by improved Pulsinelli four-vessel occlusion （4-VO） in SD rats, neuronal injury and learning and memory damage were determined. WSXN （28, 56 and 112 mg·kg^-1） and EGB761 （80 mg·kg^-1） were orally administrated for 20 days （pre-ischemia 6 d ＋ post-ischemia 14 d）. Learning and memory improvement was assessed by place navigation and spatial probe in the Morris water maze test. Brain morphology and hippocampal neuron survival were quantified by microscopy after cresyl violet and Nissl＇s staining. Results： Forebrain ischemia/ reperfusion caused hippocampal neuronal damage and learning-memory damage. WSXN （56 and 112 mg·kg^-1） and EGB761 increased density of the survival pyramidal neurons and the numbers of Nissl＇s body in the hippocampus after transient forebrain ischemia. It dose-dependently improved the learning and memory ability of vascular dementia rats. Conclusion： WSXN exerts a significant protection against neuronal injury in the hippocampus and the damage of learning and memory in forebrain ischemic rats.