葡萄与土壤中咪鲜胺及其代谢物的残留分析

Residue Analysis of Prochloraz and Its Metabolites in Grapes and Soil

[目的]建立咪鲜胺及其代谢物在葡萄及土壤中的的残留分析方法。[方法]土壤样品用乙腈提取,葡萄样品用二氯甲烷提取,盐酸吡啶使之分解,石油醚萃取,GC-ECD检测。[结果]土壤和葡萄中的平均添加回收率分别为82.4%～97.4%、81.8%～88.3%,变异系数分别为2.09%～4.63%、4.04%～5.80%,仪器最小检出量为6×10-13g,土壤和葡萄中的最低检出质量分数均为0.01 mg/kg。[结论]该方法准确可靠、重复性高、线性关系良好、分离效果好,满足残留分析要求,可用于葡萄和土壤中咪鲜胺及其代谢物的残留分析。

An analytical method for determining prochloraz and its metabolites residues in grapes and soil was established. [Methods] Prochloraz residues were abstracted by acetonitfile in soil, dichloromethane in grapes, hydrolyzed with pyridine hydrochloride, extracted with light petroleum, and determined by GC-ECD. [Results] The average recoveries in soil and grapes were 82.4-97.4% and 81.8-88.3%, and the coefficient of variation were 2.09-4.63% and 4.04-5.80% respectively. The limit of detection of 2,4,6-trichlorophenol was 6×10^-13 g, the limit of quantification was 0.01 mg/kg. [Conclusions] This method was accurate with good repeatability, good linear correlations and good separation. The results met to the requirements of pesticide residues analysis. So it could be applied for determining prochloraz and its metabolites residues in grapes and soil.