维拉帕米对静力负荷大鼠骨骼肌线粒体功能的影响

Effect of Verapamil on the Function of Mitochondria in Rats' Skeletal Muscle under Static Load

目的:探讨维拉帕米对静力负荷大鼠骨骼肌损伤的保护性作用。方法:无特定病原体性Wister大鼠24只随机分为对照组、负荷组和干预组。每组8只。干预组在实验前30 min,按10 mg/kg左下肢肌肉注射维拉帕米。实验前用2%苯巴比妥钠40 mg/kg腹腔注射麻醉,剥离大鼠左下肢比目鱼肌,将远端游离,给予100 g负荷,每组负荷90 min。对照组以同样的方式处理,不施加负荷。观察血清肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH)及骨骼肌线粒体Ca2+浓度、Ca2+-Mg2+-ATPase和丙二醛(malondialdehyde,MDA)含量变化。结果:负荷组与对照组比较,血清CK、线粒体Ca2+浓度、MDA含量明显升高、Ca2+-Mg2+-ATPase活力下降,差异有统计学意义。干预组与负荷组比较,线粒体Ca2+浓度、MDA含量明显降低,差异有统计学意义。结论:长时间静力负荷作用于骨骼肌,可导致骨骼肌损伤,维拉帕米可降低线粒体钙超载及氧化损伤作用。

Objective：To discuss the protection of verapamil on the skeletal muscle injury induced by static load.Methods： 24 healthy male Wistar rats were divided into control group,load group and intervention group respectively（8 rats every group）.Intervention group was injected verapamil im at the dose of 10 mg/kg before experiment 30 min.3 groups were anesthetized by intraperitoneal injection of 2% Phenobarbital sodium at the dose of 40 mg/kg.Distal end of soleus of lower left limb was isolated and given 100 g load for 90 min but control group was not given load.The serum creatine kinase（CK）,lactate dehydrogenase（LDH） and mitochondrial Ca2＋ concentration,Ca2＋-Mg2＋-ATPase and malondialdehyde（MDA）were observed.Results： The serum CK and mitochondrial Ca2＋ concentration,MDA content in load group were higher,but Ca2＋-Mg2＋-ATPase activity was lower than those in control group.There was significant difference.The mitochondrial Ca2＋ concentration and MDA content in intervention group were lower than those in load group.There was significant difference.Conclusion： Long time static load on the skeletal muscle can cause muscle injury.Verapamil can decrease mitochondrial Ca2＋ overload and oxidative damage induced by static load.