老年乳腺癌患者外周血趋化因子及其受体的表达

Expression of chemoklnes and their receptors in peripheral blood from elderly patients withbreast cancer

目的研究外周血趋化因子及其受体表达变化在老年乳腺癌发病机制中的作用。方法应用双抗体夹心酶联免疫吸附试验（EusA）检测30例老年乳腺癌患者（乳腺癌组）及30例健康老年人（对照组）血清活化后可调节正常T细胞表达和分泌因子（RANTES）、巨噬细胞炎性蛋白3d（MIP-3α）及间质细胞衍生因子1（SDF-1）的含量，应用流式细胞仪分析外周血CD3＋T细胞表面CC趋化因子受体（CCR）5、CCR6及CXC趋化因子受体（CXCR）4的表达。结果乳腺癌组血清RANTES、MIP-3α及SDF-1含量明显高于对照组[（27．9±16．6）μs／L比（12．6±4．2）μg/L、（10．9±7．1）μs／L比（5．44-3．5）μg，L、（339．4±94．6）ng／L比（195．0-4-70．8）μg／L，P〈0．01]；两组外周血CD3＋T细胞表面CCR5、CCR6、CXCR4表达比较差异均无统计学意义（P〉0．05）。结论外周血RANTES、MIP-3Ot及SDF-1异常升高可能在老年乳腺癌患者发病及进展过程中发挥作用。

Objective To study the role of chemokines and their receptors in peripheral blood in the pathogenesis of breast cancer in elderly patients. Methods Serum levels of regulated on activation normal T-ceil expressed and secreted （RANTES）, macrophage inflammatory protein-3 ct （MIP-3 α ） and stromal cell derived factor- 1 （SDF- 1 ） were determined by double-antibody sandwich enzyme-linked immunosorbent assay （ELISA） in 30 elderly patients with breast cancer （breast cancer group） and 30 normal controls （control group）. Meanwhile,chemokine receptors, CC chemokine receptor （CCR）5,CCR6 and CXC chemokine receptor（CXCR） 4 on peripheral blood CD3＋ T-cell in two groups were analyzed by flow cytometry. Results Serum levels of RANTES,MIP-3 ot and SDF-1 in breast cancer group were significantly higher than those in control group [ （27.9 ± 16.6） μ g/L vs. （ 12.6±4.2） Ix girL, （ 10.9 ± 7.1 ） μ g/L vs. （5.4±3.5 ） μg/L, （339.4 ±94.6） μg/L vs. （195.0±70.8） μg/L,P 〈0.01]. There was no significant difference in expressions of CCR5, CCR6 and CXCR4 on peripheral blood CD3＋ T-cell between two groups （P 〉 0.05）. Conclusion The abnormal increase of serum levels of RANTES, MIP-3 α and SDF-1 in peripheral blood may play role in the carcinogenesis and progress of breast cancer in elderly patients.