造血干细胞捐献者动员采集效果和不良反应的观察

Study of healthy donor for hematopoietic stem cell transplantation mobilized by recombinant human granulocyte-colony stimulating factor

目的:探讨中华骨髓库(CDMP)造血干细胞捐献者接受重组人粒细胞集落刺激因子(rhG-CSF)动员后外周血造血干细胞(PBSC)的采集效果及不良反应,关注CDMP志愿者rhG-CSF有关的不良反应。方法:分析108名正常供者接受rhG-CSF动员后的症状、体征、PBSC采集总量、外周血白细胞计数、CD34+细胞比例和肝功能等实验室指标变化及其恢复情况。结果:108名供者接受(5～10)μg/kg体重的rhG-CSF动员后,均获得良好的动员效果。104经1次采集,4经2次采集PBSC达到受体需要的移植细胞量的要求。动员、采集期间供者可耐受动员药物引起的不良反应。但外周血白细胞和ALT的恢复正常的时间最长者达30d。结论:rhG-CSF可有效动员正常供者的造血干细胞,但应严格采集前的体检和实验室检查,以保证中华骨髓库志愿者造血干细胞捐献的正常进行。

Objective： To investigate the adverse reactions of the healthy donors mobilized with recombinant human granulocyte colony stimulating factor（rhG-CSF）. Method：A mobilization course in 108 donors for hemato poietic stem cell transplantation（HSCT） was retrospectively studied. Result：All of the donors treated with rhG-CSF （5-10）ug/kg given subcutaneously for five days were well mobilized and the desired CD34＋ cells for HSCT were obtained after one or two aphereses. The adverse reations of rhG-CSF were well tolerated during the mobilization course in the 108 donors. Conclusion： It was confirmed that rhG-CSF could mobilize the stem cell or progenitor cell to peripheral blood effectively to meet the requirements of HSCT.