不规则抗体筛查在临床输血中的分析应用

Study of Screening Irregular Antibodies in Clinical Transfusion

目的:分析不规则抗体筛查在临床输血中的应用,降低和避免溶血性输血反应的发生。方法:采用DiaMed微柱凝胶免疫技术(IgG卡)对20000例临床住院申请输血患者标本进行不规则抗体筛查,不规则抗体阳性的标本进行抗体鉴定,同时用DiaMedIgG卡进行交叉配血。分析阳性检出者对临床配血的影响。结果:临床住院申请输血患者标本不规则抗体阳性83例,阳性率0.42%。不规则抗体血型系统的鉴定,Rh系统53例,占63.86%;MNSs系统22例,占26.50%;抗-A13例,占3.61%;P系统1例,占1.20%;Lewis系统3例,占3.61%;Kell系统1例,占1.20%。83例不规则抗体阳性标本均用DiaMedIgG卡进行交叉配血,患者均输注了无相应抗原的红细胞。结论:不规则抗体的筛查,对不规则抗体阳性标本鉴定红细胞抗体,能有效降低和避免溶血性输血反应的发生,保证临床输血安全。

Objective：To explore the significance of screening irregular antibodies in clinical transfusion to de crease or avoid hemolytic transfusion reaction. Method： Irregular antibody screening of 20000 samples were detected with Diamed micro column IgG card. Irregular antibody identification of the positive samples was detected and done cross-match with DiaMed micro column IgG card. The specificity and the frequency of occurrence were ana- lyzed and summarize. Result： 83 samples were positive of irregular antibody and positive rate was 0.42 %. Accord- ing to the detection of the blood group system of the irregular antibodies,Rh system was 52 cases, accounting for 62. 6 5% MNSs system 20 cases, accounting for 24.10 %; anti A1 4 cases, accounting for4.82 %; P system 2 cases, accounting for 2.41%; Lewis system 3 cases, accounting for 3.61%; and Kell system 2 cases, accounting for 2.41%. Conclusion： Screening of irregular antibodies in patients could decrease or avoid hemolytic transfusion reac tion, which would help to ensure the safety of transfusion.