产生抗D的RhD阴性者D基因及抗体变化的分析

Characteristics of D gene and antibody level of Rh negative individuals

目的:了解RhD血清学阴性的个体抗体产生的D基因基础及体内抗体的水平。方法:RhD血清学确认采用抗人球蛋白试验;抗D效价检测采用试管凝集法;RHD基因检测采用PCR-SSP法。结果:3名无偿献血者的RhD血清学检测确认均为阴性,RHD基因外显子D1～7、D9/1227G、D10、270A、1227A进行分型,结果均无阳性条带出现;4年的抗D抗体检测均大于1:8以上。结论:本文中产生抗D的个体均为RhD表型阴性、D基因缺失的个体;抗D一经产生在体内存在是长期的。

Objective.. To assess the characteristics of D gene and the level of antibody among Rh negative indi- viduals. Method：Indirect antiglobulin test（serological test）was used to identify Rh status among voluntary blood donors. The tube haemagglutination was used to test anti-D level of individuals. RhD molecular genetic testing was performed by PCR-SSP. ResulG Three volunteer donors were confirmed as RhD negative by the serological test. Result from the genetic typing of RhD gene exons D1--7, D9/1227G, D10,270A, 1227A showed no positive bands. The levels of RhD antibody were higher than 1 - 8 in 4 years. Conclusion： The production of antibodies might be due to the individuals without RhD gene. The anti-D in the body would be long lasting once it is pro- duced.