柳州地区Rh(D)阴性无偿献血者表型分布情况的调查

Study of the phenotype of distribution in the Rh-D negative blood donors of Liuzhou

目的:为了了解柳州地区Rh阴性无偿献血的情况,确保临床输血安全。方法:通过直接抗球蛋白试验和间接抗球蛋白试验,分别检测无偿献血者红细胞上有无D抗原,利用吸收放散试验检测Rh阴性无偿献血者弱Del型,用IgM抗-E、抗-e、抗-C、抗-c分型血清分别检测Rh(D)阴性者红细胞的Rh表型。结果:抗-D血清检测阴性的无偿献血者中仍能检出44例Del型,占Rh(D)阴性无偿献血人群的18.03%。200例Rh阴性无偿献血者中ccdee的表型频率为63%、Ccdee的表型频率为26%、CCdee的表型频率为5.50%;ccdEe的表型频率为4.50%,ccdEE的表型频率为1%。结论:排除Rh阴性无偿献血中的Del型,并对Rh阴性个体开展C、c、E、e抗原筛查工作,建立完整的Rh阴性无偿献血者资料库,确保临床用血安全。

Objective：TO study the RhD negative Voluntary blood donation status in Liuzhou,To ensure the safety of transfusion. Method： The D antigen was detected by direct antiglobulin test and indirect antiglobulin test from the blood donors,Weak De phenotype were identified by absorption and elution method among the RhD nega- tive blood donors, the E, e,C, c antigens were detected by using IgM anti-E, anti e, anti C, anti c reagent using di rect antiglobulin technique. Result：Among 244 the RhD negative samples,there are 44 De1 samples were found u sing adsorption and elution methods, and the positive rate was 18.03% （44/244）. The frequency of cedee was 63%,Ccdee was 26%,CCdee was 5.50%,ccdEe was 4.50% and ccdEE was 1 % from 200 the RhD negative＇ do- nors. Conclusion：Exclude the RhD negative blood donation of De1 type,and the RhD negative individuals the C, c, E,e ,et al antigens screening,the establishment of a complete RhD negative voluntary blood donors database, en sure the safety of clinical blood use.