摘要
观察了胶原蛋白在牛蛙皮肤组织中的分布形态。分别采用酸法和酸酶法提取得到酸溶胶原(ASC)和酶溶胶原(PSC)。理化性质分析结果表明:所提取得到的胶原为未变性Ⅰ型胶原;紫外吸收峰在233 nm处;ASC的变性温度(32.60℃)高于PSC的变性温度(32.22℃),而ASC的等电点(6.79)低于PSC的等电点(7.15);牛蛙皮胶原的氨基酸组分中,亚氨基酸的含量较牛皮的低,较鱼皮的高,同时,牛蛙皮胶原中含有牛皮胶原中没有的半胱氨酸。
Distribution pattern of collagen in the bullfrog skin was observed in this study.Acid-soluble collagen（ASC） and pepsin-soluble collagen（PSC） were extracted from the bullfrog skin by acetic acid and acetic acid-pepsin,respectively.Results of the studies on the physicochemical properties of ASC and PSC indicated that the collagen gained was undenatured type Ⅰ collagen.Ultraviolet absorption spectra showed that the absorption peak of bullfrog collagen was 233 nm.The denaturation temperature of ASC（32.60 ℃） was higher than that of PSC（32.22 ℃）,whereas the isoelectric point of ASC（6.79） was lower than that of PSC（7.15）.The imino acid content of collagen from bullfrog skin was lower than that from calf skin and higher than that from fish skin.Moreover,cysteine,which was not existed in calf skin collagen,was observed in bullfrog skin collagen.
出处
《皮革科学与工程》
CAS
北大核心
2012年第5期5-8,19,共5页
Leather Science and Engineering
基金
国家自然科学基金资助项目(21076129)
关键词
牛蛙皮
胶原
提纯
表征
bullfrog skin
collagen
extraction
characterization
