MHV-3诱导的暴发性肝炎小鼠肝脏表达IL-9免疫细胞的变化

Increased percentage of hepatic IL-9-secreting lymphocytes in MHV-3-induced fulminant hepatitis in mice

目的研究MHV-3病毒感染所致的暴发性肝炎小鼠肝脏T淋巴细胞亚群胞内细胞因子IL-9表达的变化,以探讨IL-9在该疾病模型中的作用。方法利用MHV-3病毒诱导Balb/cJ小鼠暴发性肝炎模型,采用流式细胞术检测肝脏分泌IL-9的淋巴细胞亚群比例的变化。结果随着MHV-3感染时间的延长,肝脏分泌IL-9的CD4+T细胞比例由感染前的1.87±1.36%于48小时升高到4.77±0.42%,差异显著(P<0.05);而分泌IL-9的CD4-CD8(-DN)T细胞比例则于感染后72小时达峰值7.83±3.30%,显著高于感染前(3.57±1.82,P<0.05)和24小时时(3.60±0.85,P<0.05)。结论随着感染时间延长,肝脏分泌IL-9的CD4+T和CD4-CD8(-DN)T淋巴细胞显著升高,说明IL-9可能参与了MHV-3诱导的暴发性肝炎小鼠免疫诱导的肝脏损伤发病过程。

Objective To investigate the changes of hepatic IL-9-secreting lymphocytes in MHV-3-induced fulminant hepatitis in mice for the analysis of involvement of IL-9 in the mechanisms of immune-mediated liver injury.Method MHV-3-induced fulminant hepatitis model was established in mice,and the percentage of hepatic IL-9-secreting T lymphocytes was determined by flow cytometry.Results The percentage of hepatic IL-9-producing CD4＋T cells increased from 1.87±1.36% to 4.77±0.42% at 48h after viral inoculation（P0.05）,while the percentage of hepatic CD4-CD8-（DN） T cells who also expressed IL-9 at 72h after infection was 7.83±3.30%,which was significantly higher than that at 24h（P0.05）.Conclusion The increased expression of IL-9 from hepatic CD4＋T and DNT lymphocytes may contribute to immune-mediated liver injury in MHV-3-induced fulminat hepatitis in mice.