补肾中药对过度训练大鼠运动能力及下丘脑室旁核c-fos表达的影响

Effects of Herbs Invigorating Kidney on the Sports Ability and C-fos Expression within PVN in Over Trained Rats

目的:观察中药雪芙蓉胶囊对雄性大鼠力竭跑台时间、血清激素水平及下丘脑室旁核c-fos表达的影响。方法:将30只雄性成年大鼠随机分为3组,即安静对照组、训练组、中药训练组。中药训练组每天灌服雪芙蓉胶囊(5mg/kg),8周后测试大鼠力竭跑台时间,检测血清睾酮及皮质醇含量,用免疫组织化学结合图像半定量方法对c-fos神经元的数量、面积及灰度进行测量和分析。结果:与训练组比较,中药训练组大鼠力竭跑台时间延长、血清睾酮水平增高(P<0.01,P<0.05)、血清皮质醇水平下降(P<0.05);下丘脑室旁核c-fos免疫阳性细胞数量和阳性产物面积表达均降低(P<0.05,P<0.01),灰度没有显著差异(P>0.05)。结论:中药雪芙蓉胶囊可提高大鼠的运动能力,延缓中枢疲劳,机理可能与此中药提高力竭运动大鼠血清睾酮水平,同时降低大鼠血清皮质醇水平及下丘脑室旁核c-fos的过度表达有关。

Objetive： To explore the effects of Xuefurong capsule on the time of exhausted treadmill, the serum steroid level and expres- sion of c - fos within paraventricular nucleus （PVN） of brain in the male rats. Methods： 30 SD rats were randomly divided into 3 groups ： the sedentary control group, the training group, the Chinese - medicine and training group. The Chinese - medicine and train- ing group were forced to taking Xuefurong capsule every day （5mg/ kg） and lasted 8 weeks. Then the time of exhausted treadmill of rats were tested, the serum testosterone and the cortisol were examined, c -fos expression within PVN was detected and analysised by using ABC immunohistochemical technique and semi quantitative. Re- suits： Compared with the training group, the time of exhaustedtreadmill was prolonged （P 〈 0. 01 ）, the serum testosterone in- creased （ P 〈 0.05 ） and the serum cortisol decreased （ P 〈 0.05 ） ; the amount and area of c - fos within PVN obviously decreased （ P 〈0.05, P 〈0.01 ）, the gray degree was not significant different （ P 〉0.01 ） in the Chinese - medicine and training group. Conclusion： Taking Xuefurong capsules may enhance the sports ability, its mech- anism might be that Xuefurong capsules increase the serum testoster- one and decrease the serum cortisol and excessive expression of c - fos within PVN at the same time.