长期递增负荷运动中雌性大鼠骨超微结构的变化

Long-term Incremental load Exercise in Female Rats Bone Ultrastructure Changes

目的:长期递增负荷运动中雌性大鼠骨超微结构的变化的影响。方法将200只3月龄经过17周递增负荷训练后,利用透射电镜(TEM)和酶联免疫吸附法检测和观察长期递增负荷跑台运动对雌性大鼠股骨的超微结构的影响、测试分析骨形成和骨吸收生化标志物的变化。结果:(1)根据大鼠动情周期抑制率BUN、体重、BMD等指标的检测结果可判断出本实验动物模型建造成功。(2)透射电镜(TEM)实验结果表明,4、9周时的运动训练对大鼠骨超微结构无明显影响,13、15、17周大强度递增负荷运动对大鼠骨超微结构影响显著,定性比较,超微结构损伤较同期对照组差异显著;(3)生化指标变化趋势表明随着运动强度的加大骨处于高转换状态,结合BMD结果判断骨吸收大于骨形成。结论:实验中运动性动情周期抑制检查BUN、BMD变化结果证实,随着运动强度的加大,大鼠动情周期显著紊乱,明显抑制了下丘脑垂体性腺轴的正常生理功能。9周前的运动组大鼠骨超微结构较同期对照组无显著差异,证实了适宜的运动强度对大鼠骨组织有较好的促进作用,9周后大强度递增负荷的运动对大鼠股骨超微结构的负面作用显著;大鼠骨超微结构的异常具有位点特异性。17周大强度递增负荷的运动导致大鼠骨处于高转换状态,骨吸收占据优势。

The purpose of long - term incremental changes in load exercise in female rats bone ultrastructure. 200 3 months after a 17 week incremental exercise, the use of transmission electron micros- copy （TEM） observation of long - term increments Treadmill Exer- cise on the ultrastructure of the female rat femur, test and analysis of bone formation and resorption biochemical markers the changes. The results of BUN, （ 1 ） according to the estrous cycle inhibition rate of body weight, BMD, and other indicators of test results can determine the success of this experimental animal models of con-struction. （2） transmission electron microscopy （ TEM）, experi- mental results show that 4,9 weeks of exercise training had no sig- nificant effect of rat bone uhrastructure, 13,15,17 Zhou Daqiang incremental exercise on the uhrastructure of rat bone significant qualitative comparative ultrastructural damage than the same period in the control group significantly different; （3）Biochemical indica- tors of changes in trends suggest that as exercise intensity increased bone in the high conversion state, combined with the BMD results to judge bone resorption than bone formation. Conclusion The experi- mental Menstrual Cycle check BUN and sports, the BMD change confirmed, as the exercise intensity increased, the rat estrous cycle significantly disordered, obviously inhibit the normal physiological function of the hypothalamus - pituitary - gonadal axis. Bone ultra- structure of the nine weeks before the exercise group than the control group over the same period no significant differences, confirming the appropriate exercise intensity on rat bone tissue has a good, high - intensity incremental movement of the load in rats after 9 weeks the negative effects of the femur uhrastructure; exception with site - specific bone ultrastructure in rats. 17 weeks incremental movement of the load in high conversion lead to rat bone resorption advantage.