葛花枳椇子及其配伍采用水提和醇提对酒精性肝损伤大鼠肝脏功能的影响

Effects of Flos Puerariae and Seeds of Hovenia Dulcis Thunb and Compatibility Groups Extracted by Water and Alcohol on Experimental Alcoholic Liver Disease of Rats

为了观察葛花、枳椇子不同比例配伍对酒精性肝损伤大鼠肝脏功能的影响,同时探讨葛花枳椇子及其配伍组采用不同的提取方法即水提和醇提是否会对药物的疗效产生影响,将Wistar大鼠,按体重随机分为空白组,模型组,东宝肝泰对照组,葛花组,枳椇子组,葛花枳椇子配伍1:1,1:2和2:1(质量比)8组,连续灌胃8周,于8周末取材,麻醉后腹主动脉取血,摘取肝脏,称重计算肝脏指数,同时检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)的含量。结果显示,造模成功,各个给药组对于肝功能均有一定的改善作用,同时可以看出水提的效果整体上优于醇提。由此表明,大量饮酒可以导致肝脏受到损伤,葛花与枳椇子不同比例配伍对酒精性肝损伤大鼠肝脏具有一定的保护作用,且实验中采用的药物应用传统水提的效果优于醇提。

In order to observe the antioxidant effect of different proportions offlos puerariae lobatae and hoveniae semoveniae semen extracted by alcohol and water, at the same time to determine whether different methods of extraction will have different effects and which method is better, alcoholism rat models were induced by administrating wine and Wistar rats were randomly divided into the normal group, the model group, the positive group, the flos puerariae group, the group of seeds of hovenia dulcis Thunb, and the groups of different ratios of compatibility. All rats were killed and their livers and blood were taken to examine the serum alanine aminotransferase （ALT）, the serum aspartate aminotransferase （AST） and the Alkaline Phosphatase Kit （ALP）. It is shown that the model test is successful and each treatment group sees a certain improvement of the liver function, and the water extraction on the whole is better than the alcohol extract as far as the effect is concerned. It is suggested that the heavy drinking can cause liver damage, the Gehua with the seeds of hovenia different proportion on the alcoholic liver injury in the rat liver has a protective effect, and the traditional water extraction of the drug application used in the experiment is better than the alcohol one.