高效液相色谱法测定抗病毒颗粒中射干苷、绿原酸和苦参碱

Determination of belamcandin,chlorogenic acid and matrine in Kangbingdu Granules by HPLC

目的采用反相高效液相色谱法,建立抗病毒颗粒(鱼腥草、川射干、山豆根、忍冬藤、板蓝根、贯众、白芷、重楼和青蒿)中射干苷、绿原酸和苦参碱的定量测定方法。方法 Diamonsil C18(200 mm×4.6 mm,5μm)色谱柱,乙腈-0.4%磷酸溶液(20∶80)(射干苷)、甲醇-0.4%磷酸溶液(35∶65)(绿原酸)和乙腈-0.1%磷酸溶液(用三乙胺调节pH值至8.0)(20∶80)(苦参碱)为流动相,检测波长265 nm(射干苷)、327 nm(绿原酸)和210 nm(苦参碱),柱温30℃,体积流量0.8mL/min。结果射干苷、绿原酸和苦参碱分别在0.04～2.01μg(r=0.999 9)、0.08～4.05μg(r=0.999 9)和0.20～5.01μg(r=0.999 8)范围内呈良好线性关系;平均回收率分别为100.6%(RSD为1.64%)、99.9%(RSD为1.86%)和99.0%(RSD为1.81%)。结论本法简便可行,结果准确,无干扰,可用于抗病毒颗粒的质量控制。

AIM To establish the quantitative method of analyzing belamcandin,chlorogenic acid and matrine in Kangbingdu Granules（Houttuyniae Herba,Iridis tectori Rhizoma,Sophorae tonkinensis Radix et Rhizoma,Lonicerae japonicae Caulis,Isatidis Radix,Dryopteridis crassirhizomatis Rhizoma,Angelicae dahuricae Radix,Paridis Rhizoma,Artemisiae annuae Herba） by HPLC.METHODS The Diamonsil C18 column（4.6 mm×200 mm 5 μm） was used.The mobile phases were acetonitrile-0.4% phosphoric acid solution（20∶ 80）（belamcandin）,methanol-0.4% phosphoric acid solution（35∶ 65）（chlorogenic acid）,acetonitrile-0.1% phosphoric acid solution（20∶ 80,adjusted to pH 8.0 with triethylamine）（matrine）.The detection wavelengths were set at 265 nm for belamcandin,327 nm for chlorogenic acid and 210 nm for matrine.The flow rate was 0.8 mL/min and the column temperature was maintained at 30 ℃.RESULTS The linear ranges of belamcandin,chlorogenic acid and matrine were 0.04-2.01 μg（r=0.999 9）,0.08-4.05 μg（r=0.999 9） and 0.20-5.01 μg（r=0.999 8）,res-pectively.The average recoveries were 100.6%（RSD of 1.64%）,99.9%（RSD of 1.86%） and 99.0%（RSD of 1.81%）,respectively.CONCLUSION This method is convenient,accurate and specific,and can be used for the quality control of Kangbingdu Granules.