从形态及功能研究MNNG诱导GES-1永生化细胞的恶性转化

Morphology and function of the malignant transformation of GES-1 of immortalized cell induced by MNNG

目的探讨甲基硝基亚硝基胍(methyl nitro nitrosoguanidine,MNNG)作用于GES-1永生化细胞所发生的恶性转化。方法 MNNG作用于GES-1永生化细胞24 h后,通过显微镜和软琼脂集落形成实验观察细胞形态学、染色体变化和集落形成情况。统计采用t检验。结果经MNNG处理的GES-1细胞(命名为MC)核增大,畸形,核染色质变粗,核仁肥大,核分裂。染色体可见双倍及多倍体,染色体断裂。克隆形成实验可见细胞接触抑制消失克隆形成,t=5.139,P<0.05。结论经MNNG诱导的GES-1细胞的生长特性改变,呈现肿瘤细胞的形态学及生长特征。

【Objective】To discuss the action of MNNG on the GES-1 of immortalized cell malignant transformation.【Methods】The effect of MNNG on GES-1 immortalized cells after 24 hours was observed by microscope and soft agar colony forming experiment of cell morphology,chromosome variation and colony formation.Statistical t-test was applied.【Results】GES-1 cells（named as MC） treated by MNNG treatment,the nuclear enlargement,malformation,nuclear staining qualitative coarse,nucleolar hypertrophy and nuclear fission appeared..Double chromosomes were visible and polyploid,chromosome was breakage.Colony forming experiment discovered cell contact inhibition disappearing and clone formation,t =5.139,P0.05.【Conclusion】The MNNG induced GES-1 cell growth to change characteristics,showing tumor cell morphology and growth characteristics.