杨柳田头菇B交配型基因信息素受体片段的克隆与分析

Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)

利用简并PCR及DNA步移法,从杨柳田头菇Agrocybe salicacola YAASM0711菌株中扩增得到了一个4231bp的核酸片段。经过比对及序列预测,所获得序列中含有杨柳田头菇交配型编码基因中的信息素受体部分,其序列长度为1194bp,包含4个内含子,5个外显子的长度分别为217bp,113bp,67bp,138bp,449bp。拼接后的ORF全长984bp,编码327个氨基酸残基。该序列与灰盖鬼伞Coprinus cinerea、双色蜡蘑Laccaria bicolor信息素受体氨基酸序列较为相似,含有7个跨膜区。信息素受体遗传进化分析显示,其与多个物种信息素受体聚集在一起,可能与真菌信息素受体的多种起源有关。

A 4 231 bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques. The result of alignment and structure prediction of DNA sequences showed that one 1 194 bp nucleotides gene ASRcbl encoding B mating pheromone re- ceptor was found, including four introns （72 bp, 49 bp, 48 bp and 41 bp） and five extrons （217 bp, 113 bp, 67 bp, 138 bp and 449 bp）. The spliced open reading frame （ORF） contains 984 bp nueleotides encoding 327 amino acid residues, and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors. The genetic evolution of pheromone receptor showed ASRcbl was clustered with more receptors, which suggested multiple ways of evolution occurred in fungi.