HPLC法同时测定开胸顺气丸中橙皮苷、和厚朴酚与厚朴酚的含量

Simultaneous Content Determination of Hesperidin,Honokiol and Magnolo in Kaixiong Shunqi Pills by HPLC

目的:建立同时测定开胸顺气丸中橙皮苷、和厚朴酚与厚朴酚含量的方法。方法:采用高效液相色谱法。色谱柱为Agi-lentZORBAXSB-C18柱,流动相为甲醇(A)-5%冰醋酸溶液(B)(梯度洗脱),流速为1.0mL·min-1,柱温为40℃,进样量为10μL,检测波长0～20min为284nm、20～65min为294nm。结果:橙皮苷进样量在0.1717～4.292μg范围内(r=0.9998)、和厚朴酚进样量在0.04004～1.001μg范围内(r=0.9994)、厚朴酚进样量在0.09280～4.640μg范围内(r=0.9998)与各自峰面积积分值均呈良好的线性关系;平均回收率分别为99.88%、99.34%、99.34%,RSD分别为0.67%、1.10%、0.86%(n=6)。结论:本法简便、准确、重复性好、精密度高,可更好地控制开胸顺气丸的质量。

OBJECTIVE： To establish the method for the content determination of hesperidin, honokiol and magnolol in Kaixiong shunqi pills. METHODS： HPLC method was adopted. The separation were achieved on an Agilent ZORBAX SB-C18 column with methanol （A）-5% acetic acid solution （B） as mobile phase （gradient elution） at the flow rate of 1.0 mL·min^-1. The column temperature was 40 ℃ and the detection wavelengths were 284 nm at 0～20 rain and 294 am at 20～65 rain. The injection volume was 10 μL. RESULTS： The linear range was 0.171 7～4.292 μg for hesperidin （r=0.999 8）, 0.04 004～1.001 μg for honokiol （r= 0.999 4） and 0.092 80～4.640 μg for magnolol （r=0.999 8） ; the average recoveries were 99.88% （RSD=0.67%, n=6）, 99.34% （RSD=1.1%, n=6） and 99.34% （RSD=0.86%, n=6）, respectively. CONCLUSION： The method is simple, accurate, reproducible and precise, and it is suitable for the quality control of Kaixiong shtmqi pills.