朱砂根抑制α-葡萄糖苷酶与抗氧化活性研究

α-Glucosidase Inhibitory and Antioxidant Activity of Ardisia crenata

对朱砂根抑制α-葡萄糖苷酶与抗氧化活性进行研究。利用96微孔板法筛选α-葡萄糖苷酶抑制活性;采用DPPH、ABTS和FRAP方法分析抗氧化活性。结果表明,乙酸乙酯部位抑制α-葡萄糖苷酶的活性最高(IC50=39.27μg/mL),石油醚部位次之(IC50=56.11μg/mL),正丁醇部位活性最弱(IC50=62.05μg/mL),但均远大于阳性对照Acarbose(IC50=1081.27μg/mL);乙酸乙酯部位抗氧化能力最强,正丁醇部位次之。乙酸乙酯部位清除DPPH自由基(IC50=38.55 mg/L)的能力比BHT(IC50=18.71 mg/L)低1/2,清除ABTS自由基的能力(IC50=3.60 mg/L)比BHT(IC50=7.44 mg/L)强,但比BHA(IC50=1.74 mg/L)弱,还原Fe3+的能力(FRAP=512.99±6.80μmoTE/g)为BHT(FRAP=1581.68±97.41μmol TE/g)的1/3。结果显示朱砂根乙酸乙酯部位抑制α-葡萄糖苷酶和抗氧化活性最好。

To investigate the α-glucosidase inhibitory and antioxidant activity of Ardisia crenata,96-microplate-based method was used to assay α-glucosidase inhibitory activity of A.crenata and antioxidant activity was determined by the method of DPPH,ABTS,and FRAP.The results showed that the ethyl acetate extract（IC50=39.27 μg/mL） had the highest α-glucosidase inhibitory activity,the petroleum ether extract came second（IC50=56.11 μg/mL）,and the n-butanol extract was the weakest（IC50=62.05 μg/mL）.But all of them showed higher activity than that of Acarbose（IC50=1081.27 μg/mL）.The ethyl acetate extract showed the highest antioxidant activity which was higher than that of n-butanol extract.The DPPH radical scavenging activity of ethyl acetate extract（IC50=38.55 mg/L） was half of BHT（IC50=18.71 mg/L） while ABTS radical scavenging activity（IC50=3.60 mg/L） was higher than that of BHT（IC50=7.44 mg/L） and lower than that of BHA（IC50=1.74 mg/L）.It exhibited the ferric reducing antioxidant power（FRAP=512.99 ±6.80 μmol TE/g） which was almost one third of BHT（FRAP=1581.68±97.41 μmol TE/g）.The results indicated that the ethyl acetate extract of A.crenata exhibited the strongest activity of α-glucosidase inhibitory and antioxidant activities.