木豆叶醇提物对皮质酮诱导的PC12细胞损伤的保护作用

Protective Effect of Alcohol Extract of Cajanus cajan on Corticosterone-induced Lesion in Cultured PC12 Cells

建立皮质酮诱导的PC12细胞损伤模型并观察木豆叶醇提物及不同组分对皮质酮损伤PC12细胞的保护作用。以100μmol/L的皮质酮诱导PC12细胞损伤;损伤后的PC12细胞与木豆叶醇提物及不同组分孵育24h,通过形态学观察、MTT检测、LDH测定,研究各组分对皮质酮损伤PC12细胞的保护作用。结果表明,PC12细胞与皮质酮孵育48 h后细胞存活率明显降低,而LDH水平显著升高。而加入木豆叶醇提物及各组分时上述效果明显减轻,且存在明显的剂量依赖关系。从以上结果可知,木豆叶醇提物及不同组分对皮质酮损伤的PC12细胞均有保护作用,且醇提物的效果最好。

The aim of this paper was to establish the corticosterone-induced damage model in PC12 cells and study the protective activity of alcohol extracts and its fractions from Cajanus cajan on corticosterone-induced PC12 cells.PC12 cells were exposed to different concentrations of corticosterone to determine the optimum concentration of establishing the damage model,and then the injured PC12 cells were incubated with the alcohol extract and its fractions for 24 h.After that the neuroprotective effect on injured PC12 cells was investigated by morphological observation,LDH detection and MTT determination.The results showed that the survival rate of PC12 cells and the cells exposed to corticosterone（100 μmol/L） for 48 h decreased obviously,with an increase in lactate dehydrogenase（LDH） level,but the effect became weak when the alcohol extracts and its fractions from the C.cajan was added to the PC12 cells,and it was in a dose-dependent manner.The author suggests that the alcohol extract and its fractions from C.cajan can generate a neuroprotective effect on injured PC12 cells induced by corticosterone,and that of alcohol extract is the most significant.