响应面法优化超声辅助提取宣木瓜总皂苷提取工艺研究

Optimization of Ultrasound-assisted Extraction of Total Triterpenoid Saponins from Chaenomeles speciosa Using Response Surface Analysis

对宣木瓜总皂苷的超声辅助提取工艺优化进行研究。在单因素试验基础上,选择提取时间、温度、乙醇浓度和料液比为自变量,以宣木瓜总皂苷得率为响应值,采用Central Composite Design试验设计方法,研究各自变量及其交互作用对宣木瓜总皂苷提取率的影响。利用Design Expert软件得到回归方程的预测模型并进行响应面分析,确定超声辅助提取宣木瓜总皂苷的最佳条件为时间61.69 min,温度62.34℃,乙醇浓度70.49%,料液比1∶30.57 g/mL,在此条件下,总皂苷提取率达到1.55%。验证实验表明,所得模型方程能较好地预测实验结果。

Response surface methodology was used to optimize the ultrasound-assisted extraction of total triterpenoid saponins from Chaenomeles speciosa.The extraction efficiency of total triterpenoid saponins was investigated with respecting to four variables including time,temperature,ethanol concentration and liquid-to-solid ratio.On the basis of a series of one-factor-at-a-time experiments,a polynomial regression model equation was fitted by the combined use of Box-Behnken experimental design and regression analysis.By analyzing the regression model using response surface analysis,the optimum extraction conditions of total triterpenoid saponins from C.speciosa were identified as follows：61.69 min extraction time,62.34 ℃extraction temperature,70.49% ethanol concentration and 1∶ 30.57 g/mL solid-to-liquid ratio,and the extration efficiency of total triterpenoid saponins was up to 1.55% under the optimized conditions.Confirmatory experiments indicated the good prediction ability of the established model.