胰岛素强化治疗对2型糖尿病大鼠氧化应激的影响研究

The Effects of Intensive Insulin Treatment on Oxidative Stress in Type 2 Diabetic Rats

目的探讨胰岛素强化治疗对2型糖尿病(T2DM)大鼠氧化应激的影响,为临床胰岛素强化治疗提供理论依据。方法将36只Wistar大鼠随机分为正常对照组(NC组,n=12)和高脂组(HF组,n=24),将HF组大鼠造成T2DM模型后再随机分为2个亚组,即糖尿病对照组(DC组,n=12)和胰岛素治疗组(IT组,n=12)。IT组大鼠皮下注射胰岛素,NC组和DC组大鼠皮下注射等容积0.9%氯化钠溶液,疗程4周,比较3大鼠实验前后血清及肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽转移酶(GSM)水平。结果 IT组血清及肝组织中SOD、GSM较DC组升高,差异有统计学意义(P<0.05);DC组较NC组降低,差异有统计学意义(P<0.01);IT组较NC组降低,差异有统计学意义(P<0.05)。IT组血清及肝组织中MDA较DC组降低,差异有统计学意义(P<0.05);DC组较NC升高,差异有统计学意义(P<0.01);IT组较NC组升高,差异有统计学意义(P<0.05)。结论 T2DM大鼠存在脂质过氧化水平升高及抗氧化酶活性下降,胰岛素强化治疗可减轻T2DM大鼠的氧化应激损伤,其机制与改善血脂代谢紊乱,减轻脂毒性有关。

Objective Through observing the effects of intensive insulin treatment on oxidative stress in type 2 diabetic rats, we are trying to provide evidence for clinical treatment to type 2 diabetes. Methods 36 Wistar rats axe randomly divided into two groups which are normal control group （group NC, n = 12） and high fat diet group （group HF, n =24）. After rats in group HF are made to type 2 diabetic model, they are randomly divided into two groups. Rats in diabetes control group （group DC, n = 12） are treated by normal saline injection. Rats in insulin treatment group （group IT, n = 12） are treated by insulin injection. The course is 4 weeks. We eompaxe the serum and hepatic tissue SOD, MDA and GSM in three groups rats before and after experiment. Results The serum and hepatic tissue SOD and GSM in group IT are higher than group DC （P 〈 0. 05）. In group DC, they are lower than group NC （P 〈 0. 01 ）. In group IT, they axe lower than group NC （P 〈 0. 05 ）. The serum and hepatic tissue MDA in group IT is lower than group DC （P 〈0. 05）. In group DC, they higher than group NC （P 〈0. 01 ）. In group IT, they are higher than group NC （P 〈0. 05）. Conclusion The increased lipid peroxidation and the decreased antioxi- dase have oeeurrenced in type 2 diabetic rats. Insulin intensive treatment earl decrease oxidative stress in type 2 dial）erie rats. It caused by the decreased lipid noxious.