3株被孢霉的鉴定及其生物学特性研究

Identification and Biological Characteristics of Three Strains of Mortierella

从土壤中分离获得3株被孢霉,对其营养、温度、pH等生物学特性进行研究,结果表明:3个菌株间生物学特性存在显著差异。3个菌株的适宜氮源均为蛋白胨,1、2、3号菌株适宜碳源依次为葡萄糖、淀粉、蔗糖;1号菌株和3号菌株的适宜生长温度均为5℃,2号菌株的适宜生长温度为30℃;3个菌株在pH 4～9环境下均能生长,适宜pH为7。由ITS rDNA分子鉴定确定3株菌均为高山被孢霉。

The nutrition requirement, growth temperature, pH value of medium and other biological characteristics of three Mortierella strains isolated from soil were studied. The results showed that there were significant differences in biological characteristics of the three strains. The optimum nitrogen source of the three strains was peptone, while the optimum carbon sources of the strain 1, strain 2 and strain 3 was glucose, starch and sucrose respectively. The optimum growth temperature for strain 2 was 30 ℃, whereas it was 5 ℃ for strain 1 and strain 3. The three strains could all grow on media with pH value 4 to 9 although pH value 7 was more suitable. The three strains were identified to belong to Mortierella alpine by ITS rDNA sequence analysis.