黑花生衣花色苷含量测定及稳定性研究

Research of the stability and content detection of anthocyanin of black peanuts clothing

从黑花生衣中提取花色苷,并测定其含量;测定不同温度、酸碱度、光照及金属离子(Cu2+、Mn2+、Mg2+、Fe3+)对黑花生衣花色苷稳定性的影响。在提取条件为料液比1∶50、乙醇浓度60%且为酸性、温度60℃、提取时间60min时,黑花生衣色素中花色苷含量为10.3mg/g;加热对花色苷有增色作用;pH小于5.0时随着pH增大花色苷的吸光度降低,pH大于5.0时随pH增大花色苷的吸光度增加,并朝蓝色趋势渐变;pH越接近7.0,光照后花色苷保存率越高;在Mn2+、Mg2+浓度为2.00mmol/L时,花色苷吸光度最高;随Fe3+、Cu2+浓度增加花色苷的吸光度随之增加,铁离子对黑花生衣花色苷无絮凝作用。

The experiment determined the containment of anthocyanins from the black peanut and stability of the anthocyanins under different temperature,pH,illumination conditions and different concentration of metal ions （Cu2＋,Mn2＋, Mg2＋ and Fe3＋）. The anthocyanin was extracted under the optimum technological condition：ratio of material to liquid 1：50,60% ethanol as extractant,extraction temperature 60℃ and time 60min. The black peanut clothing contained 10.3mg/g anthocyanins,of which the absorbance decreased with the increasing of pH when pH lowered 5.0 but increased after the pH was higher than 5.0 and the color turned to the trend of blue gradually. Also,the closer the pH reached to 7.0,the better preservation rate of anthocyanin it had. Apparently,the absorbance stayed at the highest level when the concentration of Mn2＋ or Mg2＋ was 2.00mmol/L and it rose with the concentration of Fe3＋ or Cu2＋ increasing. No flocculation was found to the anthocyanins by Fe3＋.