夏枯草水溶性多糖乙醇分级纯化及其理化性质研究

Ethanol fractionation precipitation and physico-chemical properties of soluble polysaccharides from Prunella vulgaris Linn

研究了夏枯草水溶性多糖的理化性质。采用水提醇沉法从夏枯草果穗中得到夏枯草多糖,将所得多糖复溶于水,依次在乙醇体积分数为20%、30%、40%、50%、60%和70%的条件下分级醇沉多糖,得到PVLP-1、PVLP-2、PVLP-3、PVLP-4、PVLP-5和PVLP-6等六个多糖组分。测定各组分的糖含量、糖醛酸含量及蛋白质含量等基本理化指标,并用气相色谱法测定它们的单糖组成,同时对其进行紫外和红外光谱扫描考察其光谱性质。结果表明,PVLP-1、PVLP-2、PVLP-3、PVLP-4、PVLP-5和PVLP-6均为酸性多糖,蛋白质含量较高。六个组分都含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖,其中木糖和阿拉伯糖含量最高,甘露糖最低,不含核糖和岩藻糖,但各组分中单糖具体摩尔组成比例不同。

The physico-chemical properties of soluble polysaccharides from Prunella Vulgaris Linn were studied. Soluble polysaccharides extracted with hot water and subsequently precipitated with ethanol,were successively fractionated by different gradient concentrations of ethanol （20%, 30%,40%,50%, 60% and 70%） into six fractions, named as PVLP-1, PVLP -2, PVLP -3, PVLP-4,PVLP-5 and PVLP-6. Contents of sugar, uronic acid and protein in these fractions were determined, monosaccharide compositions of them were also analyzed by gas chromatography（GC）, UV and I R spectra analysis were run simultaneously to determine their spectroscopy properties. The result showed that all six fractions were acidic polysaccharides, with high protein content. Each of these fractions all contain rhamnose, arabinose,xylose, mannose, glucose and galactose, and xylose and arabinose were the main monosaccharides in them,the content of mannose was Iowest,fucose and ribose were not found,but the mol ratios of these monosaccharides in six fractions were different.