淀粉废水发酵产细菌纤维素发酵条件的优化

Optimization of culture conditions of producing bacterial cellulose utilizing starch wastewater

以玉米淀粉废水添加葡萄糖20g/L,玉米浆40g/L,乙醇150mL/L为发酵基质,采用单因素和正交实验设计对葡糖醋杆菌(Gluconacetobacter xylinus)发酵产细菌纤维素条件进行优化。结果表明,最佳发酵条件为:装液量80mL/250mL,pH4.0,接种量9%(V/V),温度28℃;在此条件下得到细菌纤维素产量为4.41g/L。采用傅立叶转换红外光谱FTIR验证产物为细菌纤维素,并由SEM扫描电镜观察纤维素膜表面形貌。

The culture conditions of Gluconacetobacter xylinus producing bacterial cellulose utilizing corn starch wastewater（adding glucose 20g/L,corn steep liquor 40g/L,ethanol 150mL/L） were investigated through singlefactor and orthogonal tests. The suitable culture conditions were as follows.liquid level was 80mL in 250mL triangle bottle,pH4.0,inoculation volume was 9% （VN）,culture temperature was 28℃ ,the the yield of bacterial cellulose reached the peak（4.41g/L） at this time. The bacterial cellulose was verified by FTIR,SEM was used to observe the surface pattern of bacterial cellulose membrane.