甘蔗叶多糖除蛋白工艺研究

Study on deproteinization-technology of polysaccharides in sugarcane leaf

目的:筛选甘蔗叶多糖除蛋白的最佳工艺。方法:以蛋白质清除率、多糖保留率为指标,比较Ⅱ型ZTC1+1天然澄清剂法、Sevage法、三氯乙酸法(TCA法)、三氯乙酸-Sevage法清除甘蔗叶多糖中蛋白质的效果,并通过正交实验优化出最佳工艺。结果:Ⅱ型ZTC1+1天然澄清剂能有效纯化甘蔗叶多糖,且除蛋白最佳工艺为澄清剂用量为6%/3%(B/A),温度为30℃,多糖溶液浓度为3%,时间为1h/1h。结论:Ⅱ型ZTC1+1天然澄清剂能有效清除甘蔗叶多糖中的蛋白质,且澄清效果与温度和澄清剂用量有关。

Objective：To select deproteinization-technology of polysaccharides in sugarcane leaf. Methods. These different ways to removing protein from polysaccharides in sugarcane leaf contain natural clarifying agents,Sevage method＇,TeA and TCA-Sevage method,and that were compared with the clearance rate of protein and the polysaccharide retention as indexes,and the technology was optimized by orthogonal experiment. Results：Type II ZTC1＋1 natural clarifying agents could effectively purify sugarcane leaf polysaccharides,and the optimum condition of deproteinization with type II ZTC1 ＋1 natural clarifying agents were as follows,the adding amount of natural clarifying agents（i.e B and A） were 6% and 3% respectively,the clarifying temperature was 30℃,concentration of polysaccharides was 3%（m/v）,the clarifying time（i.e B and A） were 1h and 1h respectively. Conclusion ：Type II ZTC1＋1 natural clarifying agents could effectively remove protein of sugarcane leaf polysaccharides,which was relatived to the amount of clarifying agents and temprature.