不同剂量rhBMP-2/CPC体内骨诱导活性的比较研究

Effects of Porpous CPC with Different Concentrations of rhBMP-2 on Inductive Osteogenesis in Vivo

目的:对比不同剂量rhBMP-2与多孔CPC复合后的诱导成骨效应,探讨与多孔CPC复合后的rhBMP-2的量效关系。方法:将0.5 mg/ml、1 mg/ml、2 mg/ml、3 mg/ml 4种不同剂量的rhBMP-2与多孔CPC材料复合后,植入36只小鼠双侧股部肌肉内,分别于术后1周、2周及4周取材,通过大体观察、组织学分析、形态计量学分析、荧光双标测定,观察4组诱导成骨情况。结果:植入1周,rhBMP-2与多孔CPC材料复合表现出了较明显的剂量依赖性,含有较多rhBMP-2的材料内诱导形成的骨组织也较多,但骨组织的增加并未随着rhBMP-2剂量的增加而连续递增,2 mg组和3 mg组新生骨组织含量无明显差异(P>0.05)。植入4周,新生骨组织向材料内部生长,但此时的新生骨组织面积较2周增加不显著(P>0.05)。0.5 mg组新生骨组织含量仍处于最低水平,而其它三组之间却无明显差异(P>0.05)。结论:在0.5 mg/ml-2.0 mg/ml剂量范围,与多孔CPC复合的rhBMP-2诱导成骨量与其剂量成正比,最佳剂量为2 mg/ml。

Objective： To investigate the biological effect of inductive osteogenesis of porous CPC with different dose of rhBMP-2 and the interrelation to the dose of rhBMP-2. Methods： The porous CPC with 0.5 mg/ml, 1 mg/ml, 2 mg/ml, 3 mg/ml rhBMP-2 were respectively implanted into the muscle of 36 mice. The mice were killed at 1, 2 and 4 weeks postoperation. The biological effect of inductive osteogenesis was observed by histology, morphometry and fluorescence. Results： At 1 week, the dose dependent of rhBMP-2 could be observed obviously. The better ossification was observed in the porous CPC with more rhBMP-2. But the newly formed bone did not increase continously with the increase of rhBMP-2. There were no significant difference in newly formed bone between 2 mg/ml group and 3mg/ml group （P 〉0.05）. At 4 weeks, newly formed bone had grown into the porous CPC. But there were no significant difference in the area of newly formed bone at 4 weeks compared with that at 2 weeks （P〉0.05）. The content of new bone in 0.5 mg/ml group was significantly lower than that in other groups. There were no obvious differences in content of new bone among 1 mg/1, 2 mg/1 and 3mg/ml groups （P 〉0.05）. Conclusions： The content of new bone increases with the increasing in the dose of rhBMP-2 in the dose cope of 0.5mg/ml-2.0mg/ml, and the optimal dose ofrhBMP-2 is 2.0mg/ml.