谷氨酸、NMDA和吗啡对培养大鼠星形胶质细胞内钙振荡的影响

Effect of Glutamate,NMDA and Morphine on the Intracellular Calcium Signal of Cultured Rat Astrocytes

目的:研究谷氨酸、NMDA、吗啡对原代培养的大鼠星形胶质细胞的胞内钙信号的影响及受体作用机制。方法:利用LeicaAF6000活细胞工作站,检测谷氨酸、NMDA、吗啡分别灌流前后Fura-2/AM加载的星形胶质细胞内钙信号的动态变化,进一步观察分别阻断代谢性谷氨酸受体5(mGluR5)、NMDA受体(NMDA receptor,NMDAR)和阿片μ受体对诱导的胞内钙振荡的影响。结果:谷氨酸、NMDA、吗啡均可明显升高胞内游离钙的浓度([Ca2+]i),而将其相应受体拮抗后,星形胶质细胞[Ca2+]i升高的现象可以被显著抑制。结论:离体培养的星形胶质细胞膜上存在mGluR5、NMDAR和阿片μ受体,这些受体的激活可以升高星形胶质细胞的[Ca2+]i,且这些受体依赖的[Ca2+]i的调控机制可能是星形胶质细胞与神经元交互作用的重要途径之一。

Objective： To study the effect of glutamate, NMDA and morphine on the intracellular calcium signal of cultured rat astrocytes and their possible mechanisms. Methods： Using Leica AF6000 Live Cell Workstation to detect the effects of glutamate, NMDA and morphine perfusion on the calcium signal of cultured rat astrocytes. Results： Stimulated by glutamate, NMDA or morphine perfusion, the intracellular free Ca2＋ （[Ca2＋]i） of astrocytes was increased obviously, whereas this effect was bolished by the corresponding receptor antagonists, respectively. Conclusion： The activation of mGluR5, NMDAR and u-Opioid receptor can increase [Ca2＋]i of cultured rat astrocytes, and these modulation pathways of [Ca2＋]i might play an important role in the commtmication between astrocytes and neurons.