血清胃蛋白酶原Ⅰ/Ⅱ检测对胃癌诊断及预后判断的意义

Significance of serum pepsinogenⅠ/Ⅱ detection in diagnosis and prognosis of gastric cancer

目的探讨血清胃蛋白酶原Ⅰ/Ⅱ测定对胃癌诊断及预后判断的意义。方法用酶联免疫吸附实验分别测定胃溃疡、胃癌患者及健康人胃蛋白酶原Ⅰ/Ⅱ含量,并与单抗7相关抗原进行比较。结果健康人血清胃蛋白酶原Ⅰ/Ⅱ浓度分别为(98.77±31.24)ng/mL、(38.68±13.20)ng/mL;胃溃疡患者血清胃蛋白酶原Ⅰ浓度为(250.08±71.29)ng/mL,明显高于健康人(P<0.05),胃蛋白酶原Ⅱ浓度为(31.23±6.83)ng/mL,与健康人比较差异无统计学意义(P>0.05);胃癌患者血清胃蛋白酶原Ⅰ/Ⅱ浓度分别为(31.09±10.24)ng/mL、(9.67±7.23)ng/mL,均明显低于健康人(P<0.05);胃癌术后3年以上无复发或转移者血清胃蛋白酶原Ⅰ/Ⅱ浓度分别为(129.43±51.00)ng/mL、(23.83±13.68)ng/mL,明显高于新诊断胃癌患者(P<0.05);胃溃疡、胃癌患者血清单抗7相关抗原水平离散系数均较大,与胃蛋白酶原Ⅰ水平无相关性,两者在胃癌诊断中的特异性和灵敏度分别为81.1%、56.7%及91.9%、76.7%。结论血清胃蛋白酶原水平的测定可以为胃癌的诊断、疗效观察及预后判断提供依据,并优于血清单抗7相关抗原测定。

Objective To explore the significance of serum pepsinogen（PG） Ⅰ/Ⅱ detection for the diagnosis and prognosis of gastric cancer.Methods ELISA was employed to measure the serum concentration of PG Ⅰ/Ⅱin the patients with gastrelcosis and gastric cancer and healthy people and the results were compared with monoclonal gastric cancer 7 antigen（MG-7Ag）.Results In healthy people,the serum concentration of PGⅠ/Ⅱ was（98.77±31.24）ng/mL and（38.68±13.20）ng/mL respectively.In gastrelcosis patients,the concentration of PGⅠ was（250.08±71.29）ng/mL and significantly higher than that in healthy people（P0.05）,but the PGⅡ concentration was（31.23±6.83）ng/mL with no statistical difference compared with the healthy people（P0.05）.The serum concentrations of PGⅠ/Ⅱ in the patients with gastric cancer were（31.09±10.24）ng/mL and（9.67±7.23）ng/mL respectively and both lower than those in health people（P0.05）.The serum PGⅠ/Ⅱ concentrations in gastric cancer patients without recrudescence or metastasis in postoperative more than three years was（129.43±51.00）ng/mL and（23.83±13.68）ng/mL respectively,which were significantly higher than those in newly diagnosed patients（P0.05）.The serum concentration of MG-7Ag in the patients with gastrelcosis or gastric cancer had high dispersion coefficient and had no relativity with that of PGⅠ.The specificity and sensitivity of MG-7Ag for diagnosis of gastric cancer was 81.1% and 56.7% respectively,which of PGⅠ were 91.9% and 76.7% respectively.Conclusion Serum PG detection could provide the evidence for the diagnosis,follow-up and prognosis of gastric cancer,which is better than MG-7Ag detection.