流感病毒多表位基因盒的设计与预测

Design and prediction of multi-epitope gene of influenza A virus

目的预测流感病毒抗原表位并设计合成多表位基因盒。方法收集不同类型流感病毒基因序列,联合运用Syf-peithi、ProPred、Multipred2、Bcepred、Clastal X1.83、SWISS-MODEL等多种生物信息学软件进行分析,预测流感病毒Matrix1(M1)、Matrix2(M2)、Nucleoprotein(NP)和HA蛋白上的抗原表位,设计并合成流感病毒多表位基因盒。结果在不同亚型流感病毒的HA蛋白抗原上成功筛选出2个B细胞表位,在M1、M2及NP蛋白上分别筛选出3个T细胞表位,以一定的间隔序列串联各表位,设计并合成流感多表位基因盒。预测结果显示该多表位基因盒具有良好的同源性及抗原性。结论成功设计出包含T细胞表位及B细胞表位的流感病毒多表位基因盒Eg,为流感多表位基因疫苗的研发奠定了基础。

OBJECTIVE To design and predict multi-epitope gene of influenza A virus.METHODS Different gene sequences of current popular influenza A virus were searched.The epitopes on Matrix1protein（M1）,Matrix2 protein（M2）,Nucleoprotein（NP） and Hemagglutinin（HA） were analyzed and screened using bioinformatics programs such as Syfpeithi,ProPred,Multipred2,Bcepred,Clastal X1.83 and SWISS-MODEL.The multi-epitope gene of influenza A virus was designed and synthesized by series connection.RESULTS Two B cell epitopes on HA and three T cell epitopes on M1,M2 or NP were screened and multi-epitope gene of influenza A virus was designed successfully.Prediction of epitope showed that the multi-epitope gene had better homology and antigenicity,and could keep better biologicalactivity after incised by protease.CONCLUSION The Multi-epitope gene combined with T cell epitopes and B cell epitopes,has been successfully prepared and lays the foundation for further study on the multi-epitope gene vaccine against influenza A virus.