HPLC指纹图谱对比研究川芎和当归的化学成分

Comparison of components between Ligusticum chuanxiong and Angelia sinensis by HPLC fingerprints

目的对比川芎、当归药材水溶性和脂溶性成分的HPLC指纹图谱。方法分别收集12批川芎、10批当归药材,采用HPLC建立指纹图谱;色谱条件为LUNA C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-1%冰醋酸,进行梯度洗脱,检测波长280 nm,流速1.0 mL.min-1,柱温35℃。结果分别建立了川芎、当归药材水溶性和脂溶性成分的HPLC指纹图谱共有模式。结论所建立的指纹图谱可作为区分川芎、当归药材的参考依据。

OBJECTIVE To establish a method to distinguish between Ligusticum chuanxiong and Angelcia sinensis by HPLC fingerprints of the water-soluble and fat-soluble constituents in them.METHODS The HPLC assay was used to establish the fingerprints of Ligusticum chuanxiong and Angelica sinensis,and 12 pieces（L.chuanxiong） or 10 pieces（A.Sinensis） of goods were compared.The HPLC separation was performed on LUNA C18（2）,150 mm×4.6 mm column by gradient elution with a mixture consisting of methanol and 1%HAC at a flow rate of 1.0 mL·min-1.Detection wavelength was set at 280 nm.The temperature of column was at 35 ℃.RESULTS The mutual mode of HPLC fingerprints of water-soluble and fat-soluble constituents in Ligusticum chuanxiong and Angelica sinensis were set up.CONCLUSION It is a good reference to distinguish between Ligusticum chuanxiong and Angelica sinensis.