肺癌患者血浆肿瘤型M2丙酮酸激酶的测定及其临床意义

The measurement and clinical significance of plasma tumor M2 pyruvate kinase in patient with lung carcinoma

目的探讨肿瘤型M2丙酮酸激酶(M2-PK)在肺癌患者血中的表达水平及其临床应用价值。方法用ELISA法分别检测67例肺癌(肺癌组)、30例良性肺部疾病患者(肺良性病变组)和30名健康体检者(对照组)血浆中的肿瘤型M2-PK表达水平,并进行比较分析。结果肺癌组血浆肿瘤型M2-PK水平及阳性率均高于肺良性病变组和对照组(P<0.05);肿瘤型M2-PK诊断肿瘤的敏感性为65.7%,特异性为95.0%。肿瘤直径≥3cm者血浆肿瘤型M2-PK阳性率显著高于肿瘤直径<3cm者(P<0.05);有淋巴结转移者血浆肿瘤型M2-PK的阳性率显著高于无淋巴结转移者(P<0.05);肺癌TNMⅢ～Ⅳ期患者血浆肿瘤型M2-PK的阳性率显著高于Ⅰ～Ⅱ期者(P<0.05)。结论肿瘤型M2-PK对肺癌的辅助诊断有较高的临床价值,并对临床分期、判断淋巴结转移及临床治疗具有一定的指导意义。

Objective To investigate the diagnostic values of tumor M2 pyruvate kinase（M2-PK） level in lung cancer. Methods The concentration of tumor M2-PK in plasma was detected by ELISA of 67 cases of lung cancer（ lung cancer group） ,30 cases patients with benign lung disease（ lung benign group） and 30 healthy subjects（ control group）. Compared the result of three groups. Results The tumor M2-PK concentration in plasma and the positive rate in lung cancer group were singificantly higher than those in benign lung group and control group （ P 〈 0.05 ）. The sensitivity of tumor M2-PK in diagnosis tumor was 65.7%, the specificity was 95.0%. The tumor M2-PK positive rate of tumor diameter ≥3cm was significantly higher than that of the tumor diameter 〈 3cm（P 〈0.05 ）. The plasma tumor M2-PK positive rate of patients with lymph node metastasis was significantly higher than that of patients without lymph node metastasis （P 〈 0.05 ）. The plasma tumor type M2-PK positive rate of lung cancer III - IV period was significantly higher than that of I - II period （ P 〈 0.05 ）. Conclusion The measurement of tumor M2-PK is of great clinical value for the diagnosis of lung cancer. It is helpful in the clinical stage, evaluation of lymph node metastasis, and clinical treatment.