解偶联蛋白-1基因3826A→G变异与2-型糖尿病的相关研究

Relationship between the 3826 A→G Variation of Uncoupling Protein-1 Gene and Type-2 Diabetes Mellitus

为研究解偶联蛋白 - 1(UCP- 1)基因 382 6 A→ G变异与 2 -型糖尿病发生和发展的关系 ,应用聚合酶链反应限制性片段长度多态性 (PCR- RFL P)方法检测了 83例 2 -型糖尿病患者及 74例正常对照者的 UCP- 1基因382 6 A→ G变异 ,并测定体质量指数 (BMI)、空腹血糖血脂及胰岛素。结果 :UCP- 1基因 382 6变异基因型频率与糖尿病无相关性 ;糖尿病组突变等位基因 (G)与 BMI呈正相关 (P <0 .0 5 ) ;糖尿病组中 ,G等位基因携带个体的低密度脂蛋白胆固醇 (L DL- C)水平增高和高密度脂蛋白胆固醇 (HDL- C)水平降低有显著意义 (P值分别小于 0 .0 1和 0 .0 5 )。表明 UCP- 1基因 382 6 A→ G变异与 2 -型糖尿病患者体脂增多相关 ;G等位基因可能是 2

To investigate the role of the 3826 A→G variation of uncoupling protein 1 (UCP 1) gene in the genesis and development of type 2 diabetes mellitus, UCP 1 gene 3826 A→G variation was genotyped by PCR RFLP assay in 83 type 2 diabetic subjects and 74 cases of normal control. And body mass index (BMI) as well as the levels of fasting plasma glucose, lipid profile and insulin were measured. Results:genotype frequencies of UCP 1 gene3826 A to G variation were not associated with diabetes;mutant allele (G) was significant associated with BMI(P<0 05)in diabetic subgroups;the levels of low density lipoprotein cholesterol (LDL C) were higher while the levels of high density lipoprotein cholesterol (HDL C) were lower in patients with G allele than those without the G allele in diabetic subgroups (P<0 01 and P<0 05 respectively). These findings suggest that the 3826 A to G variation in UCP 1 gene contributes to increase body fat and the G allele tends to increase the risk of coronary heart disease in type 2 diabetic subjects.