脂质过氧化大鼠脑组织超微结构改变及杧果甙对脑组织的保护作用

The Ultramicro-structure Change of Lipid Superoxided Rat Brain Tissue and Protect of Mangiferin on the Tissues

为了探讨过氧化脂质对大鼠脑组织超微结构的影响以及果甙对脑组织的保护作用。用四氧嘧啶建立脂质过氧化动物模型 ,大鼠分 :(A)空白对照组 :动物只给 5 %淀粉水溶液 10 ml/ d灌胃 2 1d;(B)果甙高剂量组 :动物每天给予果甙 30 mg/ kg灌胃 2 1d;(C)果甙低剂量组 :动物每天给予果甙 15 mg/ kg灌胃 2 1d;(D)阳性对照组 :动物每天给予维生素 E75 mg/ kg灌胃 2 1d;(E)模型组 :动物给予生理盐水 10 ml/ d灌胃 2 1d。除 A组外 ,均于实验后第 16天起每天腹腔注射四氧嘧啶 75 mg/ kg,第 2 1天将各组动物进行灌注固定 ,观察尾状核神经元超微结构改变。结果 A组动物脑组织神经元细胞器形态正常 ,结构清楚 ,突触密度数 (N v )为 (1.2 33± 0 .10 2 )个 /微米 3;B组动物脑组织神经元各项指标与 A组差别不大 ,N v为 (1.34 2± 0 .0 98)个 /微米 3;C组动物脑组织神经元线粒体嵴减少 ,嵴间隙增宽 ,突触密度数减少 ,N v为 (0 .8376± 0 .0 75 )个 /微米 3;D组动物脑组织神经元各项指标与 A组差别不大 ,但线粒体嵴增宽 ,N v为 (1.2 2 1± 0 .0 92 )个 /微米 3;E组动物脑组织神经元线粒体嵴减少 ,嵴间隙增宽 ,突触密度数明显减少 ,N v为 (0 .82 6 2± 0 .0 5 8)个 /微米 3。说明果甙具有清除过氧化脂质作用 ,减?

To investigate the ultramicro structure change of lipid superoxided rat brain tissue and protect of mangiferin on the tissues, we established a lipid superoxided model for rat using chemical substance alloxan for our study.Rats were divided into 5 groups:Group A:the animals were gastrogavaged 10 ml/day of 5% starch solution for 21 days.Group B:30 mg/kg/day of mangiferin for 21 days.Group C:15 mg/kg/day of mangiferin for 21 days.Group D:75 mg/kg/day of Vit E for 21 days.Group E:10 ml/day of saline for 21 days,and Groups B to E were also intraperitoneal injected 75 mg/kg/day of alloxan from day 16 to day 21 The animals were then executed for perfuse fixation,and ultramicro structure change in caudate nucleus of brain tissue were observed.The forms of organelles in neurons of Group A were normal, Nv:(1 233±0 102)/μm 3.,while it showed that Group B’s were roughly normal,Nv:(1 342±0 098)/μm 3.The mitocondrial cristae in neurons of Group C were reduced,the width between crista increased.Nv reduced(0 837 6±0 075).The organelles in neurons of Group D were roughly normal,but the width between crista increased,Nv:(1 221±0 092). Organelles in neurons of Group E were abnormal,cristae reduced,the width between crista increased significantly,and the Nv also reduced significantly(0 826 2±0 058).Mangiferin can scavenge lipids superoxides from brain tissue,and protect the normal function of neuron.