新疆哈萨克族、汉族RA患者HLA-DR4等位基因及其与疾病活动的关联性研究

The correlations between HLA-DR4 gene and rheumatoid arthritis in Kazaks and Han population in Xinjiang

目的:探讨新疆哈萨克族、汉族类风湿关节炎(RA)患者的遗传易感基因的特点和其与临床及实验室指标的关系。方法:使用PCR-SSP方法检测新疆哈萨克族、汉族RA患者和健康者HLA-DR4的基因频率,分析其与RA临床、实验室指标之间的关系。结果:HLA-DR4等位基因在哈萨克族、汉族RA患者中出现频率均高于同族HC组(P<0.05或0.01),哈萨克族RA患者HLA-DR4阳性率高于汉族RA患者(P<0.01)。哈萨克族、汉族RA患者DR4阳性与DR4阴性患者发病年龄、病程、肿痛关节数、DAS28评分、ESR、CRP、RF、抗环瓜氯酸肽抗体、抗角蛋白抗体未发现有差异。哈萨克族HLA-DR4阳性患者出现早期骨破坏的发生率高于HLA-DR4阴性组(P<0.01)。结论:HLADR4是新疆哈萨克族、汉族RA患者主要的易感基因,哈萨克族患者中HLA-DR4阳性与骨破坏有关。

Objective： To evaluate the correlations between HLA-DR4 gene and clinical characteristics or laboratory parameters of rheumatoid arthritis （RA） in Kazaks and Han population in Xinjiang. Methods： The frequency of HLA-DR4 allele was determined in 122 Kazaks RA patients and 103 controls using polymerase chain reaction sequence-specific （PCR-SSP） ; so was in 115 Han patients and 119 controls. Results： HLA-DR4 allele frequency was significantly higher in RA patients in both Kazaks and Han population, while it was also significant higher in Kazaks patients that in Han patients （P 〈0. 01 ）. There was no significant difference in onset time, disease dura- tion, joint swelling counts, joint pain counts, DAS28 scores, ESR, CRP, RF, CCP or AKA between DR4 positive and negative patients. However, there were significantly higher bone erosion rate on X-ray in DR4 positive Kazaks patients （P 〈0. 01 ）. Conclusions： HLA-DR4 is correlated with RA in both Kazaks and Han population in Xinjiang. Bone erosion is more frequent in DR4 positive patients in Kazaks population.