饮食诱导肥胖与肥胖抵抗大鼠糖类吸收比较

Comparison of sugar absorption in diet-induced obesity and diet-induced obesity resistance rats

目的探讨饮食诱导肥胖(DIO)大鼠与饮食诱导肥胖抵抗(DR)大鼠小肠糖类吸收功能的差异。方法高脂饲料建立饮食诱导肥胖与肥胖抵抗大鼠模型,8周后比较2组大鼠体重、摄食量和食物效能;随机选取8只大鼠分别进行口服葡萄糖耐量试验和口服D-木糖耐量试验,比较各时间点血糖浓度和血清D-木糖浓度。结果 DIO大鼠摄食量(417.8±33.5)kJ/d和食物效能(1.5±0.1)×100 g/kJ均高于DR大鼠(379.3±40.2)kJ/d、(1.3±0.1)×100 g/kJ,差异均有统计学意义(均P<0.05);口服葡萄糖耐量试验中DIO组曲线下面积[(16.5±1.2)mmol/(L.h)]>DR组[(14.6±1.5)mmol/(L.h)],差异有统计学意义(t=-2.75,P<0.05),口服D-木糖耐量试验2组大鼠曲线下面积差异无统计学意义。结论高脂饮食诱导的肥胖与肥胖抵抗大鼠对葡萄糖的吸收存在差异,这可能在肥胖形成中起到一定作用。

Objective To explore the difference in intestinal sugar-absorption between diet-induced obesity（DIO） and diet-induced obesity resistance（DR）rats.Methods The diet-induced obesity and diet-induced obesity resistance rats models were established by feeding the rats with high-fat diet.Eight weeks after the treatment,the body weight,food intake,and food efficiency were compared between two groups.In addition,8 rats were randomly chosen for oral glucose tolerance test（OGTT）and oral D-xylose tolerance test（OXT）.Glucose concentration and serum D-xylose concentration were compared at different time points before and after the administration of gavage.Results Food intake and food efficiency of DIO rats were more than those of DR rats（417.8±33.5 kJ/day vs 379.3±40.2 kJ/day,1.5±0.1×100 g/kJ vs 1.3±0.1×100 g/kJ;P0.05）.Area under the curve of DIO group was more than that of DR group in OGTT（16.5±1.2 mmol/L · h vs 14.6±1.5 mmol/L · h;P0.05）.There was no significant difference between the two groups in the area under the curve for OXT.Conclusion There are differences in glucose-absorption between high-fat diet-induced obesity and high-fat diet-induced obesity resistant rats,which may play a role in the development of obesity.