植物雌激素-木酚素提取方法建立

Extraction of phytoestrogen lignan

目的建立一种亚麻木酚素-开环异落叶松树脂酚二葡萄糖苷(SDG)的实验室提取和检测方法。方法在SDG的提取过程中对提取步骤的每一个环节进行单因素实验,观察不同提取方法、溶液及浓度、时间及碱解方法对亚麻籽SDG提取率的影响,用高效液相色谱测定SDG的含量。结果 3种不同提取方法的SDG粗提物固体得率分别为索氏提取器法2.37%、加热回流法3.04%、超声波提取法2.89%;3种不同溶剂的SDG粗提物固体得率分别为甲醇2.76%、乙醇3.04%、丙酮3.86%;在加热回流条件下,用50%的丙酮溶液提取的SDG得率最高,为5.33%;最佳提取时间为2 h;提取过程中的碱解方法采用Ca(OH)2-H3PO4系统,SDG粗提物纯度为32.48%。结论建立的亚麻籽木酚素提取方法比原方法有所改进,提取时间有所缩短。

Objective To explore a laboratory method for the extraction of secoisolariciresinol diglucoside（SDG） from flaxseed.Methods In the extraction of SDG,all factors were tested,including different extraction methods,the concentration of extract solution,the extraction time,and different alkali hydrolysis methods.Flaxseed SDG was detected by high performance liquid chromatography（HPLC）.Results The SDG extraction is the most efficient with reflux extraction method.The best solution is 50% acetone,and the extraction time is 2 hours.The alkali extraction method is suitable with Ca（OH）2-H3PO4 system.The purity of crude SDG was 32.48%.Conclusion More SDG could be extracted in short time using the method established.