摘要
以菌丝长速和菌丝干质量为测量指标,结合液体发酵培养生长曲线,研究了越橘菌根菌菌丝生长的最佳培养基和最佳培养时间。结果表明:从4种培养基中初筛出PDMA培养基为适合该菌株生长的基础培养基,得到了该菌株种子培养的最佳液体转接时间为液态发酵的第2～3天,最佳生产时间为第5天,产量最高,为3.45g/100mL。
Taking growth rate and dry weight of mycelium as measurement index,combined with the growth curve of liquid fermentation culture,PDMA medium was found suitable as basic medium for this strain after 4 kinds of medium were preliminarily screened.And the optimal liquid transfer time for culturing the seed of this strain was the 2~3 d of liquid fermentation;the best production time was the 5 d,where the yield was the highest as 3.45 g/100mL.
出处
《北方园艺》
CAS
北大核心
2012年第20期110-112,共3页
Northern Horticulture
基金
辽宁省科技攻关资助项目(2011215005)
关键词
菌根菌
种子培养基
筛选
mycorrhizal fungi
medium
fermentation
