板蓝根双糖fructopyrano-(1→4)-glucopyranose对荷瘤小鼠肿瘤生长及免疫功能的影响

Effects of Fructopyrano-(1→4)-Glucopyranose Extracted from Radix Isatidis on Tumor Growth and Immune Function in Tumor-Bearing Mice

目的探讨板蓝根双糖fructopyrano-(1→4)-glucopyranose(FG)的体内抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法建立小鼠肉瘤S180及小鼠肝癌H22移植性肿瘤模型,观察FG对荷瘤小鼠肿瘤生长的抑制作用;采用腹腔巨噬细胞吞噬实验、淋巴细胞转化实验及Elisa法测定细胞因子IL-2、IL-6、IL-12、TNF-α的水平,检测FG对荷瘤小鼠免疫功能的影响。结果在50、100、200 mg.kg-1时FG对S180的肿瘤生长抑制率分别为23.60%、36.76%、49.26%,对H22肿瘤生长抑制率分别为22.61%、36.52%、46.95%,且能提高荷瘤小鼠的脾指数和胸腺指数;FG能增强荷瘤小鼠腹腔巨噬细胞的吞噬功能,促进淋巴细胞转化反应,升高荷瘤小鼠细胞因子IL-2、IL-6、IL-12、TNF-α的水平。结论 FG有较强的体内抗肿瘤活性,其抗肿瘤机制可能与增强荷瘤机体免疫功能有关。

OBJECTIVE To study the effects of fructopyrano-（1→4）-glucopyranose （FG） extracted from Radix Isatidis on the tumor growth and immune activity in tumor-bearing mice. METHODS The antitumor activity of FG in vivo was observed by implanted H22 and S180 mouse models. The effect of FG on immune function in tumor-bearing mice was investigated by using phagocytosis test, T plymphocyte proliferation test, and Elisa for determining the expression levels of TNF-α, IL-2, IL-6, and IL-12. RESULTS At dosa- ges of 50,100 and 200 mg· kg-1, the inhibition rates of FG were 23.60% ,36.76% and 49. 26% for S180,22. 61%, 36. 52% , and 46. 95% for H22, respectively. FG increased the spleen index and chest gland index, improved the phagocytosis function of the macrophage and enhance the T lymphocytes proliferation, and increased the expression levels of TNF-α, IL-2, IL-6, and IL-12. CONCLU- SION FG exhibits significant anti-tumor effect in vivo and the mechanism may be related to enhancing immune function .