摘要
为了调查我国西藏地区无浆体的分布和流行情况,2011年5月从西藏当雄县采集蜱样511份,其中西藏革蜱(Dermacentor everestianus)66份、银盾革蜱(Dermacentor niveus)445份,以16SrRNA基因为检测目标,采用无浆体属特异性引物EE1/EE2对蜱的全基因组进行PCR扩增,对PCR阳性样本进行随机克隆、测序,经过序列比对和系统发育分析,结果发现这些阳性样品均为一种无浆体病原——羊无浆体(Anaplasma ovis)感染,且它们与在甘肃发现的无浆体具有较近的亲缘关系。PCR结果显示,这些蜱的总感染率为6.7%(34/511)。
In order to investigate the prevalence of Anaplasrna spp. in Tibetan area, 511 adult ticks, 66 Dermacentor everestianus and 445 Dermacentor niveus respectively, were collected from Dangxiong County in May 2011. All the tick DNA samples were detected by PCR targeting a 1 430 bp fragment of 16 S rRNA gene of Anaplasrna spp. with the primer set EE1/EE2. The pairwise similarities and phylogenetic rela- tionship were analyzed based on the partial 16 S rRNA sequences. Sequencing results indicated that only Anaplasma ovis was present, and its incidence was 6.7%（34/511）.
出处
《动物医学进展》
CSCD
北大核心
2012年第10期9-13,共5页
Progress In Veterinary Medicine
基金
国家"973"基金项目(2010CB530206)
关键词
羊无浆体
16
S
RRNA基因
西藏革蜱
银盾革蜱
Anaplasma ovis
16 S rRNA gene
Dermacentor everestianus lDerrnacentor niveus
