用丝素蛋白作壁材制备丝素蛋白-维生素E微胶囊的试验

An Experiment on the Preparation of Fibroin-vitamin-E Microcapsule with Silk Fibroin

以具有良好乳化性、稳定性及胶体特性的丝素蛋白溶液作为壁材,与维生素E共混后进行高温喷雾干燥,制备丝素蛋白-维生素E微胶囊。通过优化维生素E与丝素蛋白溶液的配比,提高微胶囊的成囊性能。当维生素E与1%丝素蛋白溶液的质量比为0.5∶1时,制备的微胶囊成囊性能较好,包埋率在90%以上,胶囊颗粒间大小相差不大,囊径在3～5μm之间,囊壁厚度在500 nm～1μm之间。推测丝素蛋白囊壁在高温喷雾干燥过程中其蛋白质结晶区结构发生变化,从而有利于提高对药物的缓释性能。

Silk fibroin solution has good emulsification, stability and colloidal behavior. Fibroin-vitamin-E microcapsules were prepared using silk fibroin as wall material which was mixed with vitamin E by means of high temperature spray-dr- ying. The proportion of vitamin E and silk fibroin solution was optimized to improve capsule property. When the mass ratio of vitamin E to 1% silk fibroin solution was 0.5 ： 1, the microcapsules had good performance with an encapsulating rate o- ver 90%. The difference of size between prepared microcapsules was very little, with diameter and thickness ranging from 3 to 5 μm and 500 nm to 1 μm respectively. It is suggested that the crystalline domain of silk fibroin was changed af- ter high temperature spray-drying treatment, being favorable to the slow-release of encapsulated drug.