大孔树脂富集纯化红毛五加中总苷类成分的工艺优选

Optimization of Enrichment and Purification Technology for Total Glycosides from Acanthopanax giraldii by Macroporous Resin

目的:优选大孔树脂富集、纯化红毛五加中总苷类成分的工艺条件及参数。方法:建立RP-HPLC测定红毛五加总苷类成分含量,以紫丁香苷和齐墩果酸的洗脱率为指标,考察10种不同型号大孔树脂富集、纯化总苷类成分的吸附性能和洗脱参数。结果:选取X-5型大孔树脂,优选的纯化工艺为红毛五加总苷提取液生药质量浓度0.3 g.mL-1,调节药液pH 10,用4 BV 80%乙醇洗脱。结论:X-5型大孔树脂可很好地用于富集、纯化红毛五加总苷类成分。

Objective：To optimize technology conditions and technical parameters of enrichment and purification process for total glycosides from Acanthopanax giraldii by macroporous resin.Method：A RP-HPLC method was adopted to determine the content of total glycosides in A.giraldii with elution ratio of syringin and oleanolic acid as indexes,adsorption characteristics and elution parameters of enrichment and purification technology were investigated from 10 kinds of macroporous resin.Result：X-5 was the best macroporous resin;Optimum purification technology was：the concentration of total glycosides extraction from A.giraldii 0.3 g ·mL-1,pH of liquid 10,eluted with 4 BV 80% ethanol.Conclusion：X-5 macroporous resin could be well used for enrichment and purification of total glycosides from A.giraldii.